CAJ | 학술논문

　　目的探讨靛玉红甲肟对肝癌HepG2细胞增值和凋亡的影响。方法分别用0、10、20、40μmol/L靛玉红甲肟作用于HepG2细胞24、48、72h，采用MTT法检测细胞抑制率；用0、10、20、40μmol/L靛玉红甲肟作用于HepG2细胞24h后收集样品，采用流式细胞术法检测细胞周期及其凋亡率。结果 MTT法测定靛玉红甲肟对肝癌HepG2细胞的增殖具有抑制作用，且表现出剂量和时间的依赖性，随着浓度升高时间加长，抑制明显（P＜0.01）。流式细胞术测定也表明靛玉红甲肟对肝癌HepG2细胞有诱导凋亡的作用，凋亡随着靛玉红甲肟浓度的增大不断地增加。细胞形态学方面也可观察出细胞随着靛玉红甲肟剂量和时间的增大而逐渐出现越来越多的凋亡，细胞核染色质深染，聚集于核膜下呈新月形，细胞膜突起呈小泡样，小泡脱落形成含核小体片段的凋亡小体。结论靛玉红甲肟确实可以诱导HepG2细胞凋亡，但靛玉红甲肟对肝癌细胞作用机制还不明确，需要作进一步的深入研究。
　　목적탐토전옥홍갑우대간암HepG2세포증치화조망적영향。방법분별용0、10、20、40μmol/L전옥홍갑우작용우HepG2세포24、48、72h，채용MTT법검측세포억제솔；용0、10、20、40μmol/L전옥홍갑우작용우HepG2세포24h후수집양품，채용류식세포술법검측세포주기급기조망솔。결과 MTT법측정전옥홍갑우대간암HepG2세포적증식구유억제작용，차표현출제량화시간적의뢰성，수착농도승고시간가장，억제명현（P＜0.01）。류식세포술측정야표명전옥홍갑우대간암HepG2세포유유도조망적작용，조망수착전옥홍갑우농도적증대불단지증가。세포형태학방면야가관찰출세포수착전옥홍갑우제량화시간적증대이축점출현월래월다적조망，세포핵염색질심염，취집우핵막하정신월형，세포막돌기정소포양，소포탈락형성함핵소체편단적조망소체。결론전옥홍갑우학실가이유도HepG2세포조망，단전옥홍갑우대간암세포작용궤제환불명학，수요작진일보적심입연구。
Objective To discuss the effect of indirubin-3’-monoxime on proliferation and apoptosis of HepG2 cells. Method HepG2 cells were treated with 0, 10, 20, 40μmol/L indirubin-3’-monoxime for 24、48、72hours, then the apoptosis rate was measured by methabenzthiazuron (MTT) assay, also, it was measured by flow cytometry. Use the coloring agent DAPI and PI to dye the HepG2 cells which had been treated, then observe the cell morphology under the fluorescence microscope. Results Indirubin-3’-monoxime inhibited growth of HepG2 cells in a dose-dependent and time-dependent. The apoptosis rate increased after the treatment by indurubin-3’-monoxime at 24 hours, this apoptosis are differences between different dose group. In cell morphology, we can observe that more and more cells apoptosis along with the increasing of a dose and time of indurubin-3’-monoxime.Chromatin of cell nucleus concentrating and anachromasis, gathering in nuclear membranes as crescent-shaped. Cell membranes raised as bubble. The bubble will fall off and formed apoptotic body which contain fragment of nucleosome. Conclusion Indirubin oxime can indeed induce apoptosis in HepG2 cells, but Indirubin oxime on hepatoma cell mechanism is not clear, the need for further in-depth study.