CAJ | 학술논문

采用共沉淀法制备乳酸插层MgAl 水滑石,在水中剥离后用于固载辣根过氧化物酶,制得水滑石固载酶,测试水滑石固载酶的稳定性和降解苯酚的性能。利用XRD、IR、UV-Vis等表征技术对制备的水滑石固载酶进行了表征,并对固载过程进行了分析。实验结果表明,加入磷酸缓冲溶液有利于剥离水滑石的固载,固载时剥离水滑石层板重构成磷酸根插层水滑石,而 H RP吸附在水滑石上。H RP 在水滑石上的固载过程符合拟一级动力学方程。由于水滑石具有无限延长的层板,为 H RP 的吸附提供了充足的表面积,在实验条件下,Langmuir 和Freundlich模型均较好地拟合了实验数据。H RP在水滑石上固载后,提高了其稳定性,新制备的固载酶处理苯酚的去除率在80%以上,循环使用3次后降解率依然可达50%左右。
채용공침정법제비유산삽층MgAl 수활석,재수중박리후용우고재랄근과양화물매,제득수활석고재매,측시수활석고재매적은정성화강해분분적성능。이용XRD、IR、UV-Vis등표정기술대제비적수활석고재매진행료표정,병대고재과정진행료분석。실험결과표명,가입린산완충용액유리우박리수활석적고재,고재시박리수활석층판중구성린산근삽층수활석,이 H RP흡부재수활석상。H RP 재수활석상적고재과정부합의일급동역학방정。유우수활석구유무한연장적층판,위 H RP 적흡부제공료충족적표면적,재실험조건하,Langmuir 화Freundlich모형균교호지의합료실험수거。H RP재수활석상고재후,제고료기은정성,신제비적고재매처리분분적거제솔재80%이상,순배사용3차후강해솔의연가체50%좌우。
Lactate intercalated layered double hydroxide (Lact-LDH )was prepared by co-precipitation,then it was delaminated in water.The delaminated LDH was used to immobilize horseradish peroxidase (HRP),and the stability and performance for the degradation on phenol by the resulted LDH immobilized HRP was investi-gated.It was shown that adding phosphate buffer solution was helpful to the immobilization and the delamina-ted LDHs were reconstructed to phosphate intercalated LDHs,on which surfaces the HRPs were adsorbed. The immobilization process could be described by the pseudo-first-order kinetics model.Under the experimental conditions,both Langmuir and Freundlich isotherms fitted the experimental data,owing to the infinite surfaces of LDH.The stability of HRP was improved after being immobilized on LDH.The phenol removal could reach above 80% on the as-prepared immobilized HRP and still kept 50% after 3 cycles usage.