CAJ | 학술논문

为满足观赏栽培对六道木种苗的需求，以具生长点的嫩茎为材料，采用组织培养的方法，进行了生长点的生长、生长芽的分化、分化芽的生根、试管苗的生根及生根继代增殖培养、试管苗的移栽与定植的研究。结果表明：1／2Ms＋6-BA0．8mg／L＋NAA0．1mg／L是生长点伸长生长培养的理想培养基；MS＋AgNO30．5mg／L＋ZT1．5mg／L＋NAA0．1mg／1．是生长芽分化培养与分化继代增殖培养的理想培养基；1／4MS＋蔗糖15g／L＋ABT0．9mg／L是分化芽生根培养和试管苗生根继代增殖培养的理想培养基。试管苗移栽成活率为95．6％，定植成活率为98．4％，定植的试管苗保持了六道木的植物学性状和观赏性状。
위만족관상재배대륙도목충묘적수구，이구생장점적눈경위재료，채용조직배양적방법，진행료생장점적생장、생장아적분화、분화아적생근、시관묘적생근급생근계대증식배양、시관묘적이재여정식적연구。결과표명：1／2Ms＋6-BA0．8mg／L＋NAA0．1mg／L시생장점신장생장배양적이상배양기；MS＋AgNO30．5mg／L＋ZT1．5mg／L＋NAA0．1mg／1．시생장아분화배양여분화계대증식배양적이상배양기；1／4MS＋자당15g／L＋ABT0．9mg／L시분화아생근배양화시관묘생근계대증식배양적이상배양기。시관묘이재성활솔위95．6％，정식성활솔위98．4％，정식적시관묘보지료륙도목적식물학성상화관상성상。
To meet the need of germchits for cultivation, the tender stems with growing points of Abelia biflora were used as materials to do the research by using tissue culture methods, The study was mainly on growth of growing points, differenta- tion of adventitious buds, rooting and subculturing of tube seedlings, and tube seedlings transplanting colonization. The results demonstrated that 1/2MS+6--BA0.8mg/L+NAA0. lmg/L and MS+AgNO30. 5 mg/L+ZT1. 5 mg/L+NAA 0. 1 mg/L were the he optimum medium for growth and differentation of growing points respectively. 1/4MS-i-ABT1.0mg/L+ sucrose 15g/L was the suitable medium for rooting and subculture. The transplanting survival rate of tube seedlings was 95.6% and stable planting survival rate was 98.4%. Colonization of plantlets maintained for all biological traits and ornamental traits of Abelia hi flora.