CAJ | 학술논문

在基础培养液中分别添加浓度为0,50,100,200μmol/L的VE,研究不同浓度VE对成熟情况的影响。结果表明,体外培养48h后VE浓度为50μmol/L组GV期的比率显著高于100和200μmol/L组（P〈0.05）;100μmol/L组GVBD期的比率显著低于其它组（P〈0.05）;达到MI-MII期的比率各组之间差异不显著（P〉0.05）,但100μmol/L组MI-MII期的比率相对较高。体外培养72h后,VE浓度为200μmol/L组的GV期的比率显著高于对照组和100μmol/L组（P〈0.05）,与50μmol/L组之间差异不显著（P〉0.05）;达到GVBD和MI-MII期的比率各组之间均差异不显著（P〉0.05）。因此,VE对犬卵母细胞的核成熟没有明显促进作用。
재기출배양액중분별첨가농도위0,50,100,200μmol/L적VE,연구불동농도VE대성숙정황적영향。결과표명,체외배양48h후VE농도위50μmol/L조GV기적비솔현저고우100화200μmol/L조（P〈0.05）;100μmol/L조GVBD기적비솔현저저우기타조（P〈0.05）;체도MI-MII기적비솔각조지간차이불현저（P〉0.05）,단100μmol/L조MI-MII기적비솔상대교고。체외배양72h후,VE농도위200μmol/L조적GV기적비솔현저고우대조조화100μmol/L조（P〈0.05）,여50μmol/L조지간차이불현저（P〉0.05）;체도GVBD화MI-MII기적비솔각조지간균차이불현저（P〉0.05）。인차,VE대견란모세포적핵성숙몰유명현촉진작용。
The objective is to evaluate the effect of Vitamin E supplementation on nuclear maturation of canine oocytes in vitro.There were four groups respectively with 0,50,and 200 μmol/L of Vitamin E added to the culture medium.After culture for 48 h,the 100 μmol/L group was higher than other groups,there was no any significant difference between the control groups（P〉0.05）.A combination of 100 μmol/L was significantly lower the proportion of canine oocytes in the stage of GVBD（P〉0.05）.The percentage of oocytes developed to MI-MII in the 100 μmol/L group was higher than other groups,there was no any significant difference between them（P〉0.05）.Following 72 h of IVM,200 μmol/L treated oocytes demonstrated a dramatic increase compared with the controls in the GV（P〉0.05）.There was no significant difference between the stage of GVBD and MI-MII（P〉0.05）.This study indicated that a Vitamin E supplement in IVM medium can not significantly increase nuclear maturation.