CAJ | 학술논문

建立同时测定小鼠心肌组织中ATP（三磷酸腺苷）、ADP（二磷酸腺苷）和AMP（一磷酸腺苷）含量的高效液相色谱方法。采用反相高效液相色谱模式，使用SepaxBio-C18（250ram×4．6mmID，μm，200A）色谱柱，以60mmoL·L^-1磷酸二氢钾、60mmoL·L^-1磷酸氢二钾组成的缓冲液（pH=6．68）为流动相等比洗脱，流速：O．6mL·min^-1紫外检测波长为259Nm，带宽为30nm。三种目标组分在10min内实现基线分离，平均加标回收率均在99％以上，线性范围为1-200mg·L^-1检出限分别为20、20、25ng。该法简便、准确，易于移植。
건립동시측정소서심기조직중ATP（삼린산선감）、ADP（이린산선감）화AMP（일린산선감）함량적고효액상색보방법。채용반상고효액상색보모식，사용SepaxBio-C18（250ram×4．6mmID，μm，200A）색보주，이60mmoL·L^-1린산이경갑、60mmoL·L^-1린산경이갑조성적완충액（pH=6．68）위류동상등비세탈，류속：O．6mL·min^-1자외검측파장위259Nm，대관위30nm。삼충목표조분재10min내실현기선분리，평균가표회수솔균재99％이상，선성범위위1-200mg·L^-1검출한분별위20、20、25ng。해법간편、준학，역우이식。
To establish the simultaneous determination of the content of ATP, ADP and AMP in rat myocardial tissue, HPLC was adopted. It was determined by RP-HPLC with a Sepax Bio-C18 （250 mm×4.6 mm ID,5 μm,200 A）column, and UV detector at a wave length of 259 nm（BW 30 nm）. Mobile phase was 60 mmol＇L^-1 KH2PO4 and 60 mmol.L^-1 K2HPO4 （pH=6.68） and flow rate was 0.6 mL-min^-1. The separation was achieved within 10 rain. All the calibration curves were linear in the mass concentration range of 1-200 mg.L^-1. The average recoveries of the standard additions were over 97%, and the LOD were 20,20 and 25 ng for ATP, ADP and AMP respectively. This method was proved to be reliable and reproducible.