CAJ | 학술논문

目的通过化学合成靶向Survivin的SiRNA转染CNE-2细胞株的方法来探讨以Survivin为靶点的基因干预手段对鼻咽癌细胞生长抑制作用，为鼻咽癌的基因治疗提供新的实验依据。方法通过生物合成Survivin特异性siRNA转染CNE-2细胞，采用Western blotting检测Survivin在干扰后蛋白的表达情况，并筛选出干扰效果最好的siRNA序列转染细胞进行细胞生长抑制试验（CCK-8实验）检测其对细胞生长抑制作用。结果转染siRNA后，CNE-2细胞中survivin表达明显下降，筛选出转染1号序列的实验组CNE-2细胞进行CCK-8实验，结果显示，其细胞增殖明显抑制。结论靶向Survivin的siRNA转染人鼻咽癌CNE-2细胞有效抑制鼻咽癌CNE-2细胞Survivin表达，细胞增殖受到抑制。
목적통과화학합성파향Survivin적SiRNA전염CNE-2세포주적방법래탐토이Survivin위파점적기인간예수단대비인암세포생장억제작용，위비인암적기인치료제공신적실험의거。방법통과생물합성Survivin특이성siRNA전염CNE-2세포，채용Western blotting검측Survivin재간우후단백적표체정황，병사선출간우효과최호적siRNA서렬전염세포진행세포생장억제시험（CCK-8실험）검측기대세포생장억제작용。결과전염siRNA후，CNE-2세포중survivin표체명현하강，사선출전염1호서렬적실험조CNE-2세포진행CCK-8실험，결과현시，기세포증식명현억제。결론파향Survivin적siRNA전염인비인암CNE-2세포유효억제비인암CNE-2세포Survivin표체，세포증식수도억제。
Objective To explore the inhibiting effect of siRNA transfection against Survivin on the growth of nasopharyngeal carci-noma cells. Methods The chemically synthesized siRNA against Survivin gene was transfected to CNE-2 cells;the Western blotting was applied in detecting Survivin protein expression in CNE-2 cells, and those CNE-2 cells with better interference effect of siRNA se-quences were filtered out for cell growth inhibition test (CCK-8 experiments) and their inhibiting effect on cell growth was then detect-ed. Results The expression of Survivin in CNE-2 cells after SiRNA transfection was significantly decreased; CCK-8 experiment was made to the filtered out CNE-2 cells of the 1st sequence transfected, which indicated an obvious inhibition on cell proliferation. Conclu-sions Human nasopharyngeal carcinoma CNE-2 cells with transfection of siRNA targeting at Survivincan can effectively inhibit the Sur-vivin expression of CNE-2 cells, and the cell proliferation would be inhibited as well.