CAJ | 학술논문

在室内自然降温条件下，研究了马来西亚红罗非鱼(Oreochromis mossambicus×O. niloticus)致死低温，检测了在温室越冬温度（17.8℃）和低温胁迫下开始死亡时红罗非鱼血清的生化指标。结果表明，当水温降到8.6℃时，马来西亚红罗非鱼开始死亡，死亡低温范围为8.6~6.4℃，半致死低温为7.6℃；8.6℃时，红罗非鱼血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)、碱性磷酸酶(ALP)和肌酸激酶同工酶(CK-MB)5种酶的活性显著高于17.8℃时(P<0.05)，肌酐(CREA)含量、K+浓度显著上升(P<0.05)，总蛋白(TP)、白蛋白(ALB)、球蛋白(GLO)，及Ca2+含量小幅增加，而Na+和Cl-含量均显著下降(P<0.05)，胆固醇(CHOL)和甘油三酯(TG)含量略有降低。
재실내자연강온조건하，연구료마래서아홍라비어(Oreochromis mossambicus×O. niloticus)치사저온，검측료재온실월동온도（17.8℃）화저온협박하개시사망시홍라비어혈청적생화지표。결과표명，당수온강도8.6℃시，마래서아홍라비어개시사망，사망저온범위위8.6~6.4℃，반치사저온위7.6℃；8.6℃시，홍라비어혈청중곡병전안매(ALT)、곡초전안매(AST)、유산탈경매(LDH)、감성린산매(ALP)화기산격매동공매(CK-MB)5충매적활성현저고우17.8℃시(P<0.05)，기항(CREA)함량、K+농도현저상승(P<0.05)，총단백(TP)、백단백(ALB)、구단백(GLO)，급Ca2+함량소폭증가，이Na+화Cl-함량균현저하강(P<0.05)，담고순(CHOL)화감유삼지(TG)함량략유강저。
The lethal temperature was studied in Malaysian red tilapia (Oreochromis mossambicusíO. niloticus) under indoor natural cooling, and serum biochemical indexes were tested at water temperature of 17.8℃ and beginning of death in the red tilapia. The re-sults showed that red tilapia death appeared when the temperature dropped to 8.6℃, with range from 8.6℃ to 6.4℃, and the LC50 low temperature of 7.6℃. There were significantly higher activities of ALT, AST, LDH, ALP and CK-MB at 8.6℃compared with at 17.8℃(P<0.05). The CREA level and the content of K+were found to be significantly increased (P<0.05) at 8.6℃, while TP, ALB, GLO and the content of Ca2+increased slightly. However, the contents of Na+and Cl-were significantly decreased (P<0.05), with slightly lower CHOL and TG levels.