CAJ | 학술논문

为了研究鱼类氨代谢机制，克隆了鲤(Cyprinus carpio)Rh糖蛋白家族的Rhag、Rhbg、Rhcg1基因的cDNA全序列，与人类Rh基因的氨基酸序列比对结果显示，鲤Rh糖蛋白保守性较高。系统分析表明，鲤3个Rh基因与斑马鱼（Danio rerio）Rh基因的亲缘关系较近。Rhag、Rhbg、Rhcg1基因在鲤鳃组织中表达较高，而在其它组织表达的相对量极低。比较3个基因在鳃组织的表达，Rhbg的表达量极显著高于其它2个基因。这一结果预示Rhag、Rhbg、Rhcg1与鲤的氨代谢密切相关，尤其是Rhbg基因，可能是鲤氨代谢的重要因子。
위료연구어류안대사궤제，극륭료리(Cyprinus carpio)Rh당단백가족적Rhag、Rhbg、Rhcg1기인적cDNA전서렬，여인류Rh기인적안기산서렬비대결과현시，리Rh당단백보수성교고。계통분석표명，리3개Rh기인여반마어（Danio rerio）Rh기인적친연관계교근。Rhag、Rhbg、Rhcg1기인재리새조직중표체교고，이재기타조직표체적상대량겁저。비교3개기인재새조직적표체，Rhbg적표체량겁현저고우기타2개기인。저일결과예시Rhag、Rhbg、Rhcg1여리적안대사밀절상관，우기시Rhbg기인，가능시리안대사적중요인자。
In order to study the mechanism of ammonia metabolism in fish, cDNA of three Rhesus glycoprotein genes (Rhag, Rhbg and Rhcg1) were cloned and sequenced in common carp (Cyprinus carpio), and the amino acids sequences of the three Rh genes was compared with human Rh genes. Phylogenetic tree revealed that the Rh genes of common carp had close relationship with Rh genes of zebra fish (Danio rerio). The expressions of Rhag, Rhbg and Rhcg1 were found all significantly higher in gill than in any other tissue. In gill tissue, the expression of Rhbg gene was extremely higher than Rhag and Rhcg1 gene. These findings indicated that Rhag, Rhbg and Rhcg1 gene maybe play an important role in ammonia metabolism in common carp, especially the Rhbg gene while Rhbg gene may be the major transporter of ammonia.