CAJ | 학술논문

目的：探讨睾酮对体外培养的小鼠卵巢颗粒细胞抗苗勒管激素(anti-Mullerian hormone，AMH)分泌的影响。方法小鼠卵巢颗粒细胞原代培养，添加不同浓度睾酮，ELISA法检测颗粒细胞AMH的表达。结果 ELISA法证实颗粒细胞中有AMH表达，不同浓度(10-8 mmol/L、10-7 mmol/L、10-6 mmol/L)睾酮作用24 h可促进颗粒细胞分泌AMH，且随睾酮浓度的增加、作用时间延长，AMH分泌明显增加(P＜0.01)。结论睾酮促进小鼠卵巢颗粒细胞分泌AMH，高浓度比低浓度作用更强。
목적：탐토고동대체외배양적소서란소과립세포항묘륵관격소(anti-Mullerian hormone，AMH)분비적영향。방법소서란소과립세포원대배양，첨가불동농도고동，ELISA법검측과립세포AMH적표체。결과 ELISA법증실과립세포중유AMH표체，불동농도(10-8 mmol/L、10-7 mmol/L、10-6 mmol/L)고동작용24 h가촉진과립세포분비AMH，차수고동농도적증가、작용시간연장，AMH분비명현증가(P＜0.01)。결론고동촉진소서란소과립세포분비AMH，고농도비저농도작용경강。
Objective To study the effect of testosterone on secretion of anti-Mullerian hormone (AMH) in mouse ovarian granular cells cultured in vitro.Methods Primary mouse ovarian granular cells were cultured in vitro and treated with testosterone at different concentrations. Expression of AMH in mouse ovarian granular cells was detected by ELISA.Results ELISA showed that the AMH was expressed in mouse ovarian granular cells. Twenty-four hours treatment of primary mouse ovarian granular cells with testosterone at the concentrations of 10-8 mmol/L, 10-7 mmol/L and 10-6 mmol/L up-regulated their secretion of AMH in a concentration- and time-dependent manner (P<0.01).Conclusion Testosterone up-regulates the secretion of AMH in mouse ovarian granular cells in a concentration-dependent manner.