CAJ | 학술논문

目的建立一种快速的、适用于医院临床监测的伏立康唑血药浓度分析方法,同时分析监测内容.方法采用高效液相色谱法分析艾滋病人血清,使用5μm的C18柱,流动相为1％异丙醇水溶液:乙腈(40∶60),流速1.0 ml/min,波长250 nm,进行回收率、线性关系、精密度等测试,结合病人生化检验数据,分析血药浓度意义.结果 0.2～19.8 mg/L区间内,血浆中伏立康唑线性关系良好(R2=0.9912),回收率、精密度均良好,方法专属性强.结论依本法适宜快速分析临床样品,减少临床等待时间,伏立康唑的血药浓度监测具有重要的临床价值.
목적 건립일충쾌속적、괄용우의원림상감측적복립강서혈약농도분석방법,동시분석감측내용.방법 채용고효액상색보법분석애자병인혈청,사용5μm적C18주,류동상위1％이병순수용액:을정(40∶60),류속1.0 ml/min,파장250 nm,진행회수솔、선성관계、정밀도등측시,결합병인생화검험수거,분석혈약농도의의.결과 0.2～19.8 mg/L구간내,혈장중복립강서선성관계량호(R2=0.9912),회수솔、정밀도균량호,방법전속성강.결론 의본법괄의쾌속분석림상양품,감소림상등대시간,복립강서적혈약농도감측구유중요적림상개치.
Objective To develop a rapid analysis method which is applicable for the monitoring of clinical pharmacy Voriconazole's plasma concentration,and to analyze the monitoring results at the same time.Methods We analyzed plasma from HIV-positive people by high performance liquid chromatography (HPLC),using 5 μ m of Agilent Eclipse XDB-C18 column,mobile phase of 1％ isopropyl alcohol:acetonitrile (40∶60),flow rate:1.0 ml/min,wavelength:250 nm,to carry out the recovery rate,linearity and precision tests,and combined with patients' laboratory data,to analyze the significance of blood concentration.Results Within 0.2-19.8 mg · L-1,plasma concentration of Voriconazole standed a good linear relationship(R2=0.9912),recovery rate and precision,showing the specificity of this method.Conclusion Using HPLC to monitor Voriconazole plasma concentration of HIV-positive people is a fast,accurate and efficient analysis method for clinical samples,which can reduce clinical waiting time.Voriconazole plasma concentration monitoring has important clinical value.