环球中医药
環毬中醫藥
배구중의약
GLOBAL TCM
2014年
5期
337-340
,共4页
郝晓凤%谢立科%唐由之%张志芳%镇华%肖文峥%侯乐%祁怡鑫%谢万坤%秦睿%张京%杨莉梅%王欣
郝曉鳳%謝立科%唐由之%張誌芳%鎮華%肖文崢%侯樂%祁怡鑫%謝萬坤%秦睿%張京%楊莉梅%王訢
학효봉%사립과%당유지%장지방%진화%초문쟁%후악%기이흠%사만곤%진예%장경%양리매%왕흔
干眼%逍生散颗粒剂%泪腺
榦眼%逍生散顆粒劑%淚腺
간안%소생산과립제%루선
Dry eye%Xiaosheng San Granules%Lacrimal gland
目的：研究逍生散颗粒剂对干眼小鼠模型泪腺Bax mRNA、Bcl-2 mRNA表达的影响。方法选择c57小鼠,分为空白对照组、干眼模型组、逍生散低剂量组、逍生散中剂量组、逍生散高剂量组,测定泪液分泌量和泪膜破裂时间,采用荧光定量PCR的方法检测泪腺中Bax、Bcl-2的mRNA含量。结果(1)干眼模型组的泪液分泌试验Ⅰ( SIT)、泪膜破裂时间( BUT)低于空白对照组,逍生散组的SIT、BUT均高于干眼模型组,且逍生散中剂量组的SIT、BUT均高于逍生散低剂量组和逍生散高剂量组；(2)干眼模型组泪腺组织Bcl-2的mRNA含量低于空白对照组、Bax的mRNA含量高于空白对照组,逍生散低剂量组、逍生散中剂量组、逍生散高剂量组泪腺组织Bcl-2的mRNA含量高于干眼模型组、Bax的mRNA含量低于干眼模型组；(3) SIT、BUT与泪腺中Bax的mRNA含量呈负相关、Bcl-2的mRNA含量呈正相关。结论逍生散颗粒剂能够调节干眼小鼠模型泪腺中Bcl-2、Bax的mRNA,3．6 mg/g·d中等剂量的调节作用最为明显,且Bcl-2、Bax的含量与干眼的体征存在相关性。
目的：研究逍生散顆粒劑對榦眼小鼠模型淚腺Bax mRNA、Bcl-2 mRNA錶達的影響。方法選擇c57小鼠,分為空白對照組、榦眼模型組、逍生散低劑量組、逍生散中劑量組、逍生散高劑量組,測定淚液分泌量和淚膜破裂時間,採用熒光定量PCR的方法檢測淚腺中Bax、Bcl-2的mRNA含量。結果(1)榦眼模型組的淚液分泌試驗Ⅰ( SIT)、淚膜破裂時間( BUT)低于空白對照組,逍生散組的SIT、BUT均高于榦眼模型組,且逍生散中劑量組的SIT、BUT均高于逍生散低劑量組和逍生散高劑量組；(2)榦眼模型組淚腺組織Bcl-2的mRNA含量低于空白對照組、Bax的mRNA含量高于空白對照組,逍生散低劑量組、逍生散中劑量組、逍生散高劑量組淚腺組織Bcl-2的mRNA含量高于榦眼模型組、Bax的mRNA含量低于榦眼模型組；(3) SIT、BUT與淚腺中Bax的mRNA含量呈負相關、Bcl-2的mRNA含量呈正相關。結論逍生散顆粒劑能夠調節榦眼小鼠模型淚腺中Bcl-2、Bax的mRNA,3．6 mg/g·d中等劑量的調節作用最為明顯,且Bcl-2、Bax的含量與榦眼的體徵存在相關性。
목적：연구소생산과립제대간안소서모형루선Bax mRNA、Bcl-2 mRNA표체적영향。방법선택c57소서,분위공백대조조、간안모형조、소생산저제량조、소생산중제량조、소생산고제량조,측정루액분비량화루막파렬시간,채용형광정량PCR적방법검측루선중Bax、Bcl-2적mRNA함량。결과(1)간안모형조적루액분비시험Ⅰ( SIT)、루막파렬시간( BUT)저우공백대조조,소생산조적SIT、BUT균고우간안모형조,차소생산중제량조적SIT、BUT균고우소생산저제량조화소생산고제량조；(2)간안모형조루선조직Bcl-2적mRNA함량저우공백대조조、Bax적mRNA함량고우공백대조조,소생산저제량조、소생산중제량조、소생산고제량조루선조직Bcl-2적mRNA함량고우간안모형조、Bax적mRNA함량저우간안모형조；(3) SIT、BUT여루선중Bax적mRNA함량정부상관、Bcl-2적mRNA함량정정상관。결론소생산과립제능구조절간안소서모형루선중Bcl-2、Bax적mRNA,3．6 mg/g·d중등제량적조절작용최위명현,차Bcl-2、Bax적함량여간안적체정존재상관성。
Objective To study the effect of Xiaosheng San Granules on Bax mRNA, Bcl-2 mR-NA expression in lacrimal gland of dry eye mouse model. Methods c-57 mice were enrolled and randomly divided into blank control group, dry eye model group, XiaoSheng San low dose group, XiaoSheng San middle dose group, XiaoSheng San high dose group. Then tear secretion and tear break-up time were detec-ted and Bax mRNA, Bcl-2 mRNA expression in lacrimal gland detected by fluorescence quantitative PCR. Results (1) SIT, BUT of dry eye model group were lower than those of control group;SIT, BUT of Xi-aoSheng San group were higher than those of dry eye model group;SIT, BUT of XiaoSheng San middle dose group were higher than those of low dose group and high dose group. ( 2 ) Bcl-2 mRNA levels of dry eye model group were lower and Bax mRNA levels were higher than those of control group;Bcl-2 mRNA levels of XiaoSheng San low dose group, XiaoSheng San middle dose group, XiaoSheng San high dose group were higher and Bax mRNA levels were lower than those of dry eye model group;(3) SIT, BUT were negatively correlated with Bax mRNA levels in lacrimal gland and positively correlated with Bcl-2 mRNA levels. Con-clusion Xiaosheng San Granules can regulate Bax mRNA, Bcl-2 mRNA expression in lacrimal gland of dry eye mouse model and regulating effect of 3. 6 mg/g·d middle dose is the most obvious; the contents are correlated with dry eye symptoms.
