CAJ | 학술논문

目的：建立喂养型胰岛素抵抗( insulin resistance, IR)大鼠模型,观察糖脂平对喂养型IR大鼠氧化应激的影响。方法选用体重在180~200 g的8周龄雄性SD大鼠60只,适应性喂养一周后,随机分为空白对照组15只和造模组45只。空白对照组采用普通饲料喂养,造模组采用高脂饲料喂养。8周造模成功后,将造模组随机分为：模型组、糖脂平组、罗格列酮组各15只。糖脂平组给予糖脂平按生药20 g/kg·d灌胃；罗格列酮组给予罗格列酮0．8 mg/kg·d灌胃；模型组和空白对照组灌服等量的生理盐水；末次给药后禁食12小时,用正常血糖胰岛素钳夹技术评价胰岛素敏感性,检测各组大鼠的体重、血糖、血清胰岛素水平,并留取骨骼肌,检测组织匀浆中超氧化物歧化酶( SOD)、谷胱甘肽过氧化物酶( GSH-Px)、丙二醛( MDA)、过氧化氢酶( CAT)等指标。结果糖脂平能增加胰岛素敏感性,改善胰岛素抵抗,糖脂平组的M值显著高于模型组,体重、血糖、胰岛素均低于模型组,差异有统计学意义(P<0．05),与罗格列酮组比较,体重一项有统计学意义(P<0．05),血糖与胰岛素无统计学意义(P>0．05)；糖脂平组SOD、GSH-Px、CAT水平显著高于模型组,MDA显著低于模型组,差异有统计学意义(P<0．05),与罗格列酮组比较,差异无统计学意义(P>0．05)。结论糖脂平可改善胰岛素抵抗,也可改善氧化应激,反应了糖脂平可以通过改善胰岛素抵抗的上游通路---氧化应激来提高机体代谢活力,改善胰岛素抵抗,其作用机制可能是通过改善骨骼肌中线粒体的活性氧。
목적：건립위양형이도소저항( insulin resistance, IR)대서모형,관찰당지평대위양형IR대서양화응격적영향。방법선용체중재180~200 g적8주령웅성SD대서60지,괄응성위양일주후,수궤분위공백대조조15지화조모조45지。공백대조조채용보통사료위양,조모조채용고지사료위양。8주조모성공후,장조모조수궤분위：모형조、당지평조、라격렬동조각15지。당지평조급여당지평안생약20 g/kg·d관위；라격렬동조급여라격렬동0．8 mg/kg·d관위；모형조화공백대조조관복등량적생리염수；말차급약후금식12소시,용정상혈당이도소겸협기술평개이도소민감성,검측각조대서적체중、혈당、혈청이도소수평,병류취골격기,검측조직균장중초양화물기화매( SOD)、곡광감태과양화물매( GSH-Px)、병이철( MDA)、과양화경매( CAT)등지표。결과당지평능증가이도소민감성,개선이도소저항,당지평조적M치현저고우모형조,체중、혈당、이도소균저우모형조,차이유통계학의의(P<0．05),여라격렬동조비교,체중일항유통계학의의(P<0．05),혈당여이도소무통계학의의(P>0．05)；당지평조SOD、GSH-Px、CAT수평현저고우모형조,MDA현저저우모형조,차이유통계학의의(P<0．05),여라격렬동조비교,차이무통계학의의(P>0．05)。결론당지평가개선이도소저항,야가개선양화응격,반응료당지평가이통과개선이도소저항적상유통로---양화응격래제고궤체대사활력,개선이도소저항,기작용궤제가능시통과개선골격기중선립체적활성양。
Objective To investigate the effects of Tangzhiping on oxidative stress in rats with in-sulin resistance. Methods 60 male SD rats ( weighs 180~200 g, ages 8 w) were selected and fed a week for adaptation. Then, the rats were randomly divided into blank control group ( n=15 ) and model group (n=45). Blank control group were fed with common food, while model group were fed with high fat food, last for 8 weeks. After 8 weeks the mould was successfully made, these modules were randomly divided in-to:the model group, Tangzhiping group, rosiglitazone group ( n=15 in each group) . Tangzhiping group were given Tangzhiping formula particles according to the statement 20 g/kg·d to fill in the stomach;Ros-iglitazone group were given 0. 8 mg/kg·d rosiglitazone gavage;Model group and blank control group were given the same amount of saline solution,again for 8 weeks;12hs after the last administration of fasting, levels of insulin sensitivity were evaluated by the“euglycemic hyperinsulin clamp technique”, and levels of body weight, fast plasma glucose, serum insulin , were observed, Meanwhile superoxide dismutase (SOD),glutathione peroxidase (GSH-Px)malonaldehyde(MDA), and catalase(CAT)of skeletal muscle were measured. Results The M level was significantly higher while mean body weight, blood glucose, and fasting insulin of Tangzhiping group were lower than that of model group,. The differences were statisti-cally significant (P<0. 05), with rosiglitazone group, which body weight was statistically significant (P<0. 05), blood sugar and insulin had no statistical significance (P>0. 05);The mean SOD, GSH and CAT level of Tangzhiping group is significantly higher than model group, MDA level was lower, the differences were statistically significant (P<0. 05), with rosiglitazone group statistically significant (P<0. 05). Con-clusion Tangzhiping can improve insulin resistance, and also improve oxidative stress, which reflects that Tangzhiping can enhance body’s metabolic activity through improving the upstream pathway of IR-oxidative stress, in the end improve IR. Its mechanism may be through improving skeletal muscle mitochondrial ROS.