福建分析测试
福建分析測試
복건분석측시
FUJIAN ANALYSIS & TESTING
2012年
1期
34-38
,共5页
色氨酸%快速水解法%常规前处理方法%氨基酸自动分析仪
色氨痠%快速水解法%常規前處理方法%氨基痠自動分析儀
색안산%쾌속수해법%상규전처리방법%안기산자동분석의
tryptophan%rapid hydrolysis method%conventional pre-treatment methods%amino acid analyzer.
通过提高水解温度,减少水解时间的前处理方法,用氨基酸自动分析仪测定羽毛及其降解物中色氨酸的测定值。分析结果表明,采用4mol/LLiOH、145℃、水解5h的快速水解法与常规前处理方法所测得色氨酸含量基本一致,方法回收率91%,变异系数2.24%。可缩短分析时间,且不影响分析结果的准确度。
通過提高水解溫度,減少水解時間的前處理方法,用氨基痠自動分析儀測定羽毛及其降解物中色氨痠的測定值。分析結果錶明,採用4mol/LLiOH、145℃、水解5h的快速水解法與常規前處理方法所測得色氨痠含量基本一緻,方法迴收率91%,變異繫數2.24%。可縮短分析時間,且不影響分析結果的準確度。
통과제고수해온도,감소수해시간적전처리방법,용안기산자동분석의측정우모급기강해물중색안산적측정치。분석결과표명,채용4mol/LLiOH、145℃、수해5h적쾌속수해법여상규전처리방법소측득색안산함량기본일치,방법회수솔91%,변이계수2.24%。가축단분석시간,차불영향분석결과적준학도。
The pretreatment method of increasing the hydrolysis temperature and reducing the hydrolysis time, determinatingthe tryptophan contents in the feather and its degradation products by automatic amino acid analyzer.Analysis results show that the tryptophan contents determined by 4mol/L LiOH and 5h hydrolysis at 145℃ was the same as that from conventional method.The new method obtained a recovery rate of 91% with the highest repeatable CV 2.24%.It can not only shorten the analysis time, and does not affect the accuracy of the results.
