CAJ | 학술논문

目的：以培养和非培养的方法研究文昌红树林土壤中疣孢菌的生物多样性．方法：通过疣孢菌特异性PCR和克隆文库的方法对样品中非培养的疣孢菌进行生物多样性分析；用九种选择性培养基对样品中可培养的疣孢菌进行生物多样性分析．结果：非培养方法发现文昌红树林土壤放线菌的多样性指数Shanno．Wienerindex和Chaol物种多样性指数（Scha01）分别为2．3217和74．5；培养方法分别获得3株疣孢菌和74株疣孢菌类似菌．结论：文昌红树林土壤环境中蕴藏着丰富的疣孢菌多样性资源，其中大部分为目前未培养菌．
목적：이배양화비배양적방법연구문창홍수림토양중우포균적생물다양성．방법：통과우포균특이성PCR화극륭문고적방법대양품중비배양적우포균진행생물다양성분석；용구충선택성배양기대양품중가배양적우포균진행생물다양성분석．결과：비배양방법발현문창홍수림토양방선균적다양성지수Shanno．Wienerindex화Chaol물충다양성지수（Scha01）분별위2．3217화74．5；배양방법분별획득3주우포균화74주우포균유사균．결론：문창홍수림토양배경중온장착봉부적우포균다양성자원，기중대부분위목전미배양균．
Objective: Cultivation-dependent and cultivation-independent methods can be used to gain an insight into the presence, distribution and taxonomic diversity of the genus Verrucosispora in mangrove soils from Wenchang. Methods: The biodiversity of unculture members of the genus Verrucosispora in samples was analyzed by Verrucosispora-specific polymerase chain reaction(PCR) and clone library. The biodiversity of cultivable members of the genus Verrucosispora in samples was analyzed by 9 selective isolation methods. Results: The Shannon-Wiener index (H') and Schaol richness index for unculture verrucosisporal members from mangrove soils were 2.3217 and 74.5, respectively. The results presented 74 Verrucosispora-like strains isolated from mangrove samples from Wenchang using cultivation-dependent methods. Three isolates were provisionally assigned to the genus Verrucosispora on the basis of phylogenetic analysis. Conclusion: These studies diversity of verrucosisporal species that have shown that mangrove soils contain a high are rarely recovered by cultivation-based methods.