福建分析测试
福建分析測試
복건분석측시
FUJIAN ANALYSIS & TESTING
2012年
1期
12-14,19
,共4页
鬼臼毒素%尿液%间接竞争酶联免疫吸附法
鬼臼毒素%尿液%間接競爭酶聯免疫吸附法
귀구독소%뇨액%간접경쟁매련면역흡부법
podophyllotoxin%urea%IC-ELISA
本文建立了一种利用IC-ELISA法测定尿液中的鬼臼毒素的方法。尿液样经离心沉淀,采用间接竞争酶联免疫吸附法（IC-ELISA）测定。鬼臼毒素的添加浓度在0.5 mg/L-10 mg/L时,回收率范围为78.3%-99.3%,相对标准偏差（RSD）在4.3%-11.5%之间。方法最低检测浓度为0.012μg/mL,检测范围为0.025-19.59μg/mL。方法的准确度和精密度符合残留分析要求。方法操作简捷,结果可靠稳定,比仪器方法检测成本低。
本文建立瞭一種利用IC-ELISA法測定尿液中的鬼臼毒素的方法。尿液樣經離心沉澱,採用間接競爭酶聯免疫吸附法（IC-ELISA）測定。鬼臼毒素的添加濃度在0.5 mg/L-10 mg/L時,迴收率範圍為78.3%-99.3%,相對標準偏差（RSD）在4.3%-11.5%之間。方法最低檢測濃度為0.012μg/mL,檢測範圍為0.025-19.59μg/mL。方法的準確度和精密度符閤殘留分析要求。方法操作簡捷,結果可靠穩定,比儀器方法檢測成本低。
본문건립료일충이용IC-ELISA법측정뇨액중적귀구독소적방법。뇨액양경리심침정,채용간접경쟁매련면역흡부법（IC-ELISA）측정。귀구독소적첨가농도재0.5 mg/L-10 mg/L시,회수솔범위위78.3%-99.3%,상대표준편차（RSD）재4.3%-11.5%지간。방법최저검측농도위0.012μg/mL,검측범위위0.025-19.59μg/mL。방법적준학도화정밀도부합잔류분석요구。방법조작간첩,결과가고은정,비의기방법검측성본저。
A competitive indirect enzyme-linked immunosorbent assay （IC-ELISA） for podophyllotoxin was developed byusing polyclonal antibody, and its suitability for the determination of podophyllotoxin in urea was studied. Based on the polyclonal antibodies, the method showed an Is0 value of 0.22 μg/mL with a detection limit of 0.012 μ g/mL. With the spiked concentration of 0.5 mg/L-10 mg/L, the recoveries from the podophyllotoxin-fortified urea in the assay were in the range of 78.3-99.3%. A good correlation between podophyllotoxin concentration measured by the ELISA and HPLC （R^20.9934, Y=2.456X-0.123） was obtained from linear regression analysis. These results indicate that the ELISA could be a convenient and supplemental analytical tool for monitoring podophyllotoxin in urea without previous extraction or cleanup.
