CAJ | 학술논문

为了提高猪冷冻精液品质和精子抵抗低温打击的能力,本研究以5%、10%、15%、20%和25%等不同浓度的鸵鸟卵黄作为冷冻保护剂,以20%的鸡蛋卵黄和20%的鸽蛋卵黄为对照,将冷冻-解冻后的精子活率、质膜完整率和顶体完整率作为评价指标,分析鸵鸟卵黄对猪精子的抗冷冻保护作用。结果表明：稀释液中添加20%鸽蛋卵黄时,精子活率、顶体完整率和质膜完整性分别为52.11%、55.62%和54.94%,显著高于其他组（P〈0.05）。虽然稀释液中添加15%鸵鸟卵黄时,冷冻-解冻后精子活率、顶体完整率和质膜完整率显著高于5%、10%、20%和25%鸵鸟卵黄组,但仍然显著低于稀释液中添加20%鸽蛋卵黄处理组。本研究表明,鸵鸟卵黄在冷冻过程中对猪精子具有一定的保护作用,但相对于鸽子蛋和鸡蛋卵黄效果并不理想。
위료제고저냉동정액품질화정자저항저온타격적능력,본연구이5%、10%、15%、20%화25%등불동농도적타조란황작위냉동보호제,이20%적계단란황화20%적합단란황위대조,장냉동-해동후적정자활솔、질막완정솔화정체완정솔작위평개지표,분석타조란황대저정자적항냉동보호작용。결과표명：희석액중첨가20%합단란황시,정자활솔、정체완정솔화질막완정성분별위52.11%、55.62%화54.94%,현저고우기타조（P〈0.05）。수연희석액중첨가15%타조란황시,냉동-해동후정자활솔、정체완정솔화질막완정솔현저고우5%、10%、20%화25%타조란황조,단잉연현저저우희석액중첨가20%합단란황처리조。본연구표명,타조란황재냉동과정중대저정자구유일정적보호작용,단상대우합자단화계단란황효과병불이상。
In order to improve the frozen semen quality and sperm resistance to cold shock,the different concentrations of 5%,10%,15%,20% and 25% ostrich egg yolk were added in extender as cryoprotectants,and 20% egg yolk and 20% pigeon egg yolk were control.The cryoprotective effects of ostrich egg yolk on the boar spermatozoa were analyzed and spermatozoa motility,acrosome integrity and membrane integrity after freezing-thawing were evaluated.The results indicated that the sperm motility,acrosome integrity,membrane integrity were 52.11%,55.62% and 54.94% respectively while the extender was supplied with 20% pigeon egg yolk,which was higher than that of other groups（P0.05）.Although sperm motility,acrosome integrity and membrane integrity in the extender supplemented with 15% ostrich egg yolk were significantly higher than that of 5%,10%,20% and 25% ostrich egg yolk groups,they were significantly lower than that of 20% pigeon egg yolk group.According to our study,the ostrich egg yolk could protect the boar spermatozoa during the frozen-thawed,but is not as effective as pigeon egg yolk and egg yolk.