CAJ | 학술논문

选择标记基因广泛应用于植物转基因工程中，然而这些基于除草剂和抗生素类抗性选择标记基因的利用，在转基因筛选和鉴定完成后功能多余。当前转基因植物的标记基因及相关产物对环境污染和食用安全的潜在影响，已经引起了人们的广泛关注。随着转基因技术的不断发展和人们对转基因植物和食品安全性的关注，删除转基因植物中的选择标记基因非常重要。文章着重讨论了植物转基因过程中，选择标记基因的利用，删除选择标记基因的几种方法，包括共转化法、位点特异性重组、染色体内重组、转座子介导的重组、删除叶绿体转化中选择标记基因，以及无选择标记基因植株的直接再生等。同时，还对这些技术的发展作了展望。
선택표기기인엄범응용우식물전기인공정중，연이저사기우제초제화항생소류항성선택표기기인적이용，재전기인사선화감정완성후공능다여。당전전기인식물적표기기인급상관산물대배경오염화식용안전적잠재영향，이경인기료인문적엄범관주。수착전기인기술적불단발전화인문대전기인식물화식품안전성적관주，산제전기인식물중적선택표기기인비상중요。문장착중토론료식물전기인과정중，선택표기기인적이용，산제선택표기기인적궤충방법，포괄공전화법、위점특이성중조、염색체내중조、전좌자개도적중조、산제협록체전화중선택표기기인，이급무선택표기기인식주적직접재생등。동시，환대저사기술적발전작료전망。
Selectable marker genes were widely used in the transgenic plants .However, these genes based on the re-sistance to antibiotic and herbicides are redundant since transgenic plants have been selected and identified .Due to the presence of selectable marker gene and its products , the environment pollution and food safety of transgenic plants are strongly concerned by consumers .With the development of transgenic technology , it is very important to eliminate the selectable marker gene in transgenic plants .In this review , utilization of selectable marker gene , methods of marker gene excision consisted of co-transformation , site-specific recombination , intrachromosomal recombination , recombination by transposon , removal of chloroplast marker gene , regeneration of direct marker-free plants, as well as the developmental directions of this technology were discussed .