CAJ | 학술논문

采用ISSR分子标记技术对采自中国14个省区的53个稗属植物（ Echinochloa Beauv．）样本进行分子鉴定。利用9条筛选出的引物一共扩增出137条DNA谱带，其中包括136条多态性条带，多态位点百分率为99.3％。基于ISSR标记，构建了反映各样本间遗传亲缘关系的系统聚类图。结果表明，53个样本可分为9个类群，分别对应于长芒稗（ E．caudata，5份样本）、细叶旱稗（ E．crusgalli var．praticola，8份）、短芒稗（ E． crusgalli var．breviseta，6份）、孔雀稗（ E．cruspavonis，3份）、西来稗（ E．crusgalli var．zelayensis，5份）、稗原变种（E．crusgalli）和无芒稗（E．crusgalli var．mitis，共14份）、光头稗（E．colona，2份）、硬稃稗（E．glabrescens，5份）和水田稗（ E．oryzoides，5份）。从聚类结果看，基于形态特点的中国稻区稗草属植物分类群有一定的遗传学基础，但是关于其亲缘关系和分类地位仍然值得探讨。
채용ISSR분자표기기술대채자중국14개성구적53개패속식물（ Echinochloa Beauv．）양본진행분자감정。이용9조사선출적인물일공확증출137조DNA보대，기중포괄136조다태성조대，다태위점백분솔위99.3％。기우ISSR표기，구건료반영각양본간유전친연관계적계통취류도。결과표명，53개양본가분위9개류군，분별대응우장망패（ E．caudata，5빈양본）、세협한패（ E．crusgalli var．praticola，8빈）、단망패（ E． crusgalli var．breviseta，6빈）、공작패（ E．cruspavonis，3빈）、서래패（ E．crusgalli var．zelayensis，5빈）、패원변충（E．crusgalli）화무망패（E．crusgalli var．mitis，공14빈）、광두패（E．colona，2빈）、경부패（E．glabrescens，5빈）화수전패（ E．oryzoides，5빈）。종취류결과간，기우형태특점적중국도구패초속식물분류군유일정적유전학기출，단시관우기친연관계화분류지위잉연치득탐토。
Fifty three Chinese Echinochloa samples collected from 14 provinces were classified through inter-simple sequence repeat (ISSR) markers.It was shown that 137 bands were amplified by 9 selected primers, and 136 of them were polymorphic.Based on the results, 53 samples were clustered into 9 groups, i.e.E.caudata(5 sam-ples), E.crusgalli var.praticola (8 samples), E.crusgalli var.breviseta(6 samples), E.cruspavonis(3 sam-ples), E.crusgalli var.zelayensis(5 samples), E.crusgalli and E.crusgalli var.mitis(14 samples), E.colona(2 samples), E.glabrescens(5 samples) and E.oryzoides(5 samples).The present classification of Echinochloa spe-cies in Chinese paddy fields was in accordance with morphological traits .However , their genetic relationship and sys-tematic level still needs to be further investigated .