CAJ | 학술논문

为了探讨外来入侵杂草假臭草的科学利用方法，用点滴法和熏蒸法测定了假臭草精油对小菜蛾的毒杀、拒食作用及其对小菜蛾体内羧酸酯酶和谷胱甘肽-s-转移酶活性的影响。结果表明，假臭草精油对小菜蛾幼虫有一定的毒杀作用，点滴处理48 h致死中浓度为2.102×105 mg· L -1、熏蒸处理48 h致死中浓度为1.404×105 mg· L -1；假臭草精油对小菜蛾幼虫有明显的拒食作用，12 h拒食中浓度为2.69×104 mg· L-1，处理浓度为1.00×105 mg· L-1，拒食率可达100％，不同处理时间的拒食中浓度无明显差异。假臭草精油处理小菜蛾幼虫会导致小菜蛾幼虫体内的羧酸酯酶和谷胱甘肽-s-转移酶活性的变化，但未呈现出规律性。
위료탐토외래입침잡초가취초적과학이용방법，용점적법화훈증법측정료가취초정유대소채아적독살、거식작용급기대소채아체내최산지매화곡광감태-s-전이매활성적영향。결과표명，가취초정유대소채아유충유일정적독살작용，점적처리48 h치사중농도위2.102×105 mg· L -1、훈증처리48 h치사중농도위1.404×105 mg· L -1；가취초정유대소채아유충유명현적거식작용，12 h거식중농도위2.69×104 mg· L-1，처리농도위1.00×105 mg· L-1，거식솔가체100％，불동처리시간적거식중농도무명현차이。가취초정유처리소채아유충회도치소채아유충체내적최산지매화곡광감태-s-전이매활성적변화，단미정현출규률성。
To investigate the new methods and tools of alien weeds usage , effects of essential oil extracted from Prax-elis clematidea on enzyme activities (carboxylesterase and glutathione-s-transferase) of diamondback moth (DBM), Plutella xylostella, and its toxicity by using topical methods of application and fumigation were studied in this paper . Results showed that LC 5 0 after 48 h of P.clematidea essential oil against DBM larvae was 2.102 ×10 5 mg· L-1 by topical application methed and was 1.404 ×10 5 mg· L-1 by fumigation treatment .The median antifeedant concentra-tion ( AFC50 ) of P.clematidea essential oil against DBM larvae was 2.69 ×10 4 mg· L-1 .The antifeedant rate could reach 100%when treating concentration was 1.00 ×10 5 mg· L-1 .Treating time had no significant effects on AFC 50 . The activities of carboxylesterase and glutathione-s-transferase of DBM larvae treated with P.clematidea essential oil were changed significantly but not in a regular pattern .