CAJ | 학술논문

针对玉米细菌性枯萎病和玉米内州萎焉病这两种检疫性病菌进行酶联免疫-实时荧光PCR检测研究，对该方法进行了特异性、灵敏度以及实际样品模拟试验。结果表明，该方法具有较好的特异性，检测低限在100～200 cfu· mL-1，同时可用于实际样品的检测鉴定。
침대옥미세균성고위병화옥미내주위언병저량충검역성병균진행매련면역-실시형광PCR검측연구，대해방법진행료특이성、령민도이급실제양품모의시험。결과표명，해방법구유교호적특이성，검측저한재100～200 cfu· mL-1，동시가용우실제양품적검측감정。
An immuno-capture PCR ( IC-PCR) method was studied to detect two quarantine bacterial , Pantoea stew-artii subsp.stewarii and Clavibacter michiganensis subsp.nebraskense.Specificity and sensitivity of the method were examined , and the actual sample simulation experiment was conducted .The results showed that this method was high species-specific.The minimum limit of detection is between 10-100 cfu· mL-1 .The method can also be used for detection and identification of the entry-and-exit samples .