CAJ | 학술논문

利用SRAP分子标记技术对30份菊芋资源进行了基因组多态性分析。用22对扩增稳定且清晰的具有多态性DNA条带的引物组合，进行菊芋的SRAP-PCR扩增。共扩增出160条条带，其中多态性条带114条，多态性比率为71％，表明菊芋资源在DNA水平上存在广泛差异。利用NTSYS软件统计分析出资源间遗传距离在0.04～0.42之间。应用UPGMA得出聚类结果，将30份菊芋资源划分为2大类群，第2大类群分为6个亚类群。聚类结果与传统的形态学分类略有不同，可结合两种方法为菊芋资源亲缘关系的研究提供理论基础。
이용SRAP분자표기기술대30빈국우자원진행료기인조다태성분석。용22대확증은정차청석적구유다태성DNA조대적인물조합，진행국우적SRAP-PCR확증。공확증출160조조대，기중다태성조대114조，다태성비솔위71％，표명국우자원재DNA수평상존재엄범차이。이용NTSYS연건통계분석출자원간유전거리재0.04～0.42지간。응용UPGMA득출취류결과，장30빈국우자원화분위2대류군，제2대류군분위6개아류군。취류결과여전통적형태학분류략유불동，가결합량충방법위국우자원친연관계적연구제공이론기출。
Genetic diversity was analyzed in 30 accessions of Jerusalem artichoke using SRAP markers .22 primer combinations were used for SRAP-PCR amplification of Jerusalem artichoke .160 DNA fragments were amplified from 30 samples and 114 fragments were polymorphic ( percentage of polymorphic bands was 71%) , indicating that there were wide differences at DNA level among Jerusalem artichoke resource .The genetic distance indexes of 30 cultivars based on the SRAP data varied from 0.04 to 0.42 .It suggested that the genetic diversity among resources of Jerusa-lem artichoke was high .Cluster analysis with UPGMA method showed that the 30 resources tested in this study could be divided into two groups .The second group could be divided into six sub groups .The clustering results were slight-ly different with traditional morphological classification .The above two methods could be combined to provide a theo-retical basis for the study on the Jerusalem artichoke resource relationship .