CAJ | 학술논문

鲍鱼菇口感特殊，味道鲜美，营养价值高，但产量稀少。传统的形态特征进行的分类鉴定常常带有一定的局限性和主观性，采集的野生鲍鱼菇的真实性需要进一步鉴定。文章采用一对通用引物ITS1和ITS4对野生鲍鱼菇子实体样本及其组织分离菌株的rDNA ITS片段进行PCR扩增，并克隆测序。通过GenBank数据库搜索和系统发育树构建来进行分子鉴定。结合形态特点的分析，结果显示采集的野生菇样本就是鲍鱼菇。ITS序列在鲍鱼菇不同菌株的鉴定上具有重要的价值。
포어고구감특수，미도선미，영양개치고，단산량희소。전통적형태특정진행적분류감정상상대유일정적국한성화주관성，채집적야생포어고적진실성수요진일보감정。문장채용일대통용인물ITS1화ITS4대야생포어고자실체양본급기조직분리균주적rDNA ITS편단진행PCR확증，병극륭측서。통과GenBank수거고수색화계통발육수구건래진행분자감정。결합형태특점적분석，결과현시채집적야생고양본취시포어고。ITS서렬재포어고불동균주적감정상구유중요적개치。
Pleurotus abalonus has a high nutritional value , with a very special and delicious taste .But its output is very low.There are some limitations and subjectivities to its classification and determination in terms of traditional morphological features , so that the collected wild P.abalonus should be further identified for its reality .In the stud-y, the rDNA ITS sequences of the fruiting bodies and their isolates of wild P.abalonus were amplified via PCR using ITS1 and ITS4 as primer pairs.And their sequences were sequenced by clone sequencing .Molecular identification was carried out by GenBank search and ITS phylogenetic tree analysis .Combined with analysis of morphological fea-tures, the results showed that the collected wild mushroom was P.abalones.The work indicated important value of ITS sequence in identification of the different strains of mushroom .