CAJ | 학술논문

采用PCR-SSCP技术对黑羽番鸭脂蛋白脂酶（ LPL）基因进行多态性研究，分析该基因与常规肉品质、肌肉营养成分等指标的相关性。结果表明：黑羽番鸭LPL基因第3内含子上发现了A4G突变，群体纯合度为0.5054，多态信息含量为0.3723，形成了EE、EF、FF 3种基因型。 EF型母鸭腿肌失水率显著高于FF型（ P＜0.05，下同）。 EF型公鸭腿肌中镁含量显著高于FF型。 EE型公鸭胸肌中Ala含量显著高于EF型、FF型；FF型母鸭胸肌中Asp、His含量显著高于EF型；FF型公鸭腿肌中Ser、Pro含量显著高于EE型；EE型母鸭腿肌Lys含量显著高于EF型，EF型Cys、Val含量显著高于FF型。
채용PCR-SSCP기술대흑우번압지단백지매（ LPL）기인진행다태성연구，분석해기인여상규육품질、기육영양성분등지표적상관성。결과표명：흑우번압LPL기인제3내함자상발현료A4G돌변，군체순합도위0.5054，다태신식함량위0.3723，형성료EE、EF、FF 3충기인형。 EF형모압퇴기실수솔현저고우FF형（ P＜0.05，하동）。 EF형공압퇴기중미함량현저고우FF형。 EE형공압흉기중Ala함량현저고우EF형、FF형；FF형모압흉기중Asp、His함량현저고우EF형；FF형공압퇴기중Ser、Pro함량현저고우EE형；EE형모압퇴기Lys함량현저고우EF형，EF형Cys、Val함량현저고우FF형。
The polymorphism of Lipopretein lipase ( LPL) gene was detected in black Muscovy duck by PCR-SSCP, and the correlation between LPL gene polymorphism , routine meat quality , and muscle nutrients were analyzed .It was shown that the mutation of A-G was discovered at the 4th site in intron 3.The homozygosity was 0.5054 in the whole population .The polymorphic information content was 0.3723 .The mutations formed EE , EF and FF geno-types.The water loss rate of EF female duck was significantly (P<0.05) higher than that of FF female duck .The Mg content in leg muscle of the EF male duck was significantly ( P<0.05 ) higher than that in the FF male duck . The Ala content in chest muscle of the EE male duck was significantly ( P<0.05 ) higher than those of the EF and FF male duck.The Asp and His content in chest muscles of the FF female duck were significantly (P<0.05) high-er than that of EF female duck .The contents of Ser and Pro in leg muscles of the FF male duck were significantly ( P<0.05 ) higher than those of EE male duck .The Lys content in leg muscles of the EE female duck was significantly ( P<0.05 ) higher than that of EF female duck ,and the Cys and Val contents of EF female duck were significantly ( P<0.05 ) higher than those of FF female duck .