CAJ | 학술논문

饲料样品经提取、免疫亲和柱净化，以甲醇水45∶55（V/V）为流动相，进行C18柱分离，经柱后光化学衍生，以荧光检测器定量检测4种黄曲霉毒素AFB1，AFB2，AFG1和AFG2含量。结果表明，4种黄曲霉毒素在35 min内得到良好的分离，除AFG2加标回收率略低外，AFB1，AFB2，AFG1和黄曲霉毒素总量在稻草、羊草和青贮笋壳空白样的加标回收率均在80％以上，平行样相对标准偏差均低于10.0％；AFB1、AFB2、AFG1、AFG2和黄曲霉毒素总量的检出限分别为0.4，0.2，0.4，0.2和1.2μg· kg-1。试验所建立的检测方法可实现对青粗饲料中4种黄曲霉毒素的同时提取、净化、分离与检测，方法准确简便，重复性好，灵敏度高，能满足青粗饲料中多种黄曲霉毒素快速检测要求。
사료양품경제취、면역친화주정화，이갑순수45∶55（V/V）위류동상，진행C18주분리，경주후광화학연생，이형광검측기정량검측4충황곡매독소AFB1，AFB2，AFG1화AFG2함량。결과표명，4충황곡매독소재35 min내득도량호적분리，제AFG2가표회수솔략저외，AFB1，AFB2，AFG1화황곡매독소총량재도초、양초화청저순각공백양적가표회수솔균재80％이상，평행양상대표준편차균저우10.0％；AFB1、AFB2、AFG1、AFG2화황곡매독소총량적검출한분별위0.4，0.2，0.4，0.2화1.2μg· kg-1。시험소건립적검측방법가실현대청조사료중4충황곡매독소적동시제취、정화、분리여검측，방법준학간편，중복성호，령민도고，능만족청조사료중다충황곡매독소쾌속검측요구。
The present study was conducted to establish a determination method for various aflatoxins in coarse fodder by HPLC.Aflatoxins were synchronously extracted from samples , cleaned up with immunoaffinity column , separated by elution in C18 column and analyzed by fluorescence detector after the photochemical derivation .Four kinds of af-latoxins were separated within 35 min. The recovery rate of added aflatoxin AFB1, AFB2, AFG1 and total aflatoxins from rice straw, Chinese wildrye and bamboo shell silage were all above 80.0%.But, the recovery rate of AFG2 was relatively low.The relative standard deviations (RSD) for aflatoxins in parallel samples were less than 10.0%.The limit of detection (LOD) for aflatoxin AFB1, AFB2, AFG1, AFG2 and total aflatoxins were 0.4,0.2 ,0.4, 0.2 and 1.2 μg· kg-1 , respectively .Thus, the established determination method could realize simultaneous extraction , clean up, separation, and detection of aflatoxins in coarse fodder .The method is accurate , simple, high sensitive and well repeatable , which is suitable for the rapid determination of aflatoxin in coarse fodder .