CAJ | 학술논문

目的：研究骨髓间充质干细胞在鼠心力衰竭中对CD4＋CD25＋Treg细胞的影响。方法制作动物模型，雄性SD大鼠随机分为对照组A和模型组，模型组定期用阿霉素腹腔注射，对照组A用同等剂量生理盐水腹腔注射，每周1次，共6次，最后一次注射完成后继续喂养2周，行超声心动图检测左心室收缩末期容积（ LVESV ）、左室舒张末期容积（ LVEDV ）、左室射血分数（ LVEF）；并抽血通过流式细胞仪检测Tregs细胞。在最后一次注射完阿霉素1周后，分离、培养、扩增SD大鼠骨髓间充质干细胞（BMMSCs）。再将上述存活的模型组随机分为干预组，经尾静脉移植骨髓间充质干细胞组动物，对照组B，经尾静脉给予同等剂量生理盐水，分别于移植后第4周抽血通过流式细胞仪检测Tregs细胞，并行超声心动图检测LVESV，LVEDV，LVEF。结果①对照组A，B比较，LVESV，LVEF明显减低（P＜0．05），而LVEDV明显升高（P＜0．05）。②对照组A，B比较，CD4＋CD25＋Treg细胞的百分比明显减低（ P＜0．05）。结论骨髓间充质干细胞移植后可以上调CD4＋CD25＋Treg细胞，从而抑制扩张型心肌病中的免疫反应，改善心功能。
목적：연구골수간충질간세포재서심력쇠갈중대CD4＋CD25＋Treg세포적영향。방법제작동물모형，웅성SD대서수궤분위대조조A화모형조，모형조정기용아매소복강주사，대조조A용동등제량생리염수복강주사，매주1차，공6차，최후일차주사완성후계속위양2주，행초성심동도검측좌심실수축말기용적（ LVESV ）、좌실서장말기용적（ LVEDV ）、좌실사혈분수（ LVEF）；병추혈통과류식세포의검측Tregs세포。재최후일차주사완아매소1주후，분리、배양、확증SD대서골수간충질간세포（BMMSCs）。재장상술존활적모형조수궤분위간예조，경미정맥이식골수간충질간세포조동물，대조조B，경미정맥급여동등제량생리염수，분별우이식후제4주추혈통과류식세포의검측Tregs세포，병행초성심동도검측LVESV，LVEDV，LVEF。결과①대조조A，B비교，LVESV，LVEF명현감저（P＜0．05），이LVEDV명현승고（P＜0．05）。②대조조A，B비교，CD4＋CD25＋Treg세포적백분비명현감저（ P＜0．05）。결론골수간충질간세포이식후가이상조CD4＋CD25＋Treg세포，종이억제확장형심기병중적면역반응，개선심공능。
Objective To study the effect of bone marrow mesenchymal stem cells in the mouse heart failure on CD 4 +CD25 +Treg cells.Methods Male SD rats were randomly divided into control group A (n=10) and model group(n=20),and were treated regularly with intraperitoneal injection of doxorubicin in the model group and the same dose of normal saline in the control group weekly for a total of six times.Left ventricular end systolic volume (LVESV),left ventricular end diastolic volume (LVEDV),left ventricular ejection fraction ( LVEF) and blood Tregs cells were tested by Echocardiography and FlowCytometry respectively after two -week-feeding of the last injection . SD rat bone marrow mesenchymal stem cells (BMMSCs) were isolated,cultured and amplified after 1 week of the last injection of doxorubi-cin.Then the survival of the model group were randomly divided into intervention group ,the group of transplantation of bone marrow mesen-chymal stem cells via the tail vein(n=10) and control group B with the same dose saline given by intravenous (n=10) respectively.4 weeks after transplantation blood Tregs cells were tested by Flow Cytometry ,IL-10,TGF-βwere tested through ELISA,left ventricular end systolic volume(LVESV),left ventricular end diastolic volume(LVEDV),left ventricular pressure rise and maximum rate of decline ( ±dp/dtmax), left ventricular ejection fraction were tested by Echocardiography .Results ①Compared with the control group A ,LVESV, ±dp/dtmax LVEF of control group B was significantly lower (P<0.05),while LVEDV was significantly higher (P<0.05).②The percentage of CD4 +CD25 +Treg cells of control group B and control group A was significantly lower ( P<0.05).Conclusion Bone marrow mesenchymal stem cell transplantation can increase CD 4+CD25+Treg cells and promote their cytokine expression ,thereby inhibiting the immune response of dilat-ed cardiomyopathy and improving heart function .