器官移植
器官移植
기관이식
OGRAN TRANSPLANTATION
2014年
5期
289-293
,共5页
肝移植%排斥反应%模型%大鼠%白细胞介素
肝移植%排斥反應%模型%大鼠%白細胞介素
간이식%배척반응%모형%대서%백세포개소
Livertransplantation%Rejection%Model%Rat%Interleukin
目的：比较不同品系大鼠之间肝移植排斥反应特点。方法依据大鼠不同品系分成对照组SD→SD （A组），实验组Wistar→SD （B组）、Lewis→BN （C 组）以及 DA→Lewis （D组）4个组，采用Kamada双袖套法建立大鼠原位肝移植模型。比较各组受体大鼠术后10 d血清肝功能指标[丙氨酸转氨酶（ALT）、总胆红素（TB）和白蛋白（Alb）]、血清白细胞介素（IL）-2和 IL-10水平、急性排斥反应组织病理学分级和术后平均生存时间。结果与A、B组比较，C 组和D组的血清ALT和TB明显升高， Alb 明显降低，差异均有统计学意义（均为 P＜0.05）。与 C 组比较， D组的TB明显升高，Alb明显降低，差异均有统计学意义（均为P＜0.05）。与A组比较，B、C、D组大鼠血清IL-2和IL-10水平均明显升高，C 组和D组的IL-2/IL-10亦明显升高，差异有统计学意义（均为P＜0.05）。与B组比较，C组和D组大鼠血清IL-2水平明显升高，D组的IL-2/IL-10亦明显升高，差异有统计学意义（均为P＜0.05）。与C组比较，D组大鼠血清IL-2水平明显升高，D组的IL-2/IL-10亦明显升高，差异有统计学意义（均为P＜0.05）。肝组织病理学检查显示，A组大鼠肝组织未见明显排斥反应，排斥活动性指数（RAI）评分（1.8±0.7）分；B组RAI评分（3.1±1.3）分，属轻度或不明确性排斥反应；C组RAI 评分（6.9±0.8）分，属中～重度排斥反应；D组RAI 评分（8.8±0.5）分，属重度排斥反应。各组间RAI 评分比较以及组间两两比较，差异均有统计学意义（均为P＜0.05）。A 组、B 组、C 组、D 组受体大鼠术后平均生存时间分别为（119.3±1.9）d、（116.9±8.3）d、（53.4±6.1）d、（12.1±2.4）d，A组与B组的生存时间比较差异无统计学意义，余各组两两比较差异均有统计学意义（均为P＜0.05）。结论4种组合中，大鼠DA→Lewis模型排斥反应最剧烈，Lewis→BN次之，而Wistar→SD最轻，接近于免疫耐受。
目的：比較不同品繫大鼠之間肝移植排斥反應特點。方法依據大鼠不同品繫分成對照組SD→SD （A組），實驗組Wistar→SD （B組）、Lewis→BN （C 組）以及 DA→Lewis （D組）4箇組，採用Kamada雙袖套法建立大鼠原位肝移植模型。比較各組受體大鼠術後10 d血清肝功能指標[丙氨痠轉氨酶（ALT）、總膽紅素（TB）和白蛋白（Alb）]、血清白細胞介素（IL）-2和 IL-10水平、急性排斥反應組織病理學分級和術後平均生存時間。結果與A、B組比較，C 組和D組的血清ALT和TB明顯升高， Alb 明顯降低，差異均有統計學意義（均為 P＜0.05）。與 C 組比較， D組的TB明顯升高，Alb明顯降低，差異均有統計學意義（均為P＜0.05）。與A組比較，B、C、D組大鼠血清IL-2和IL-10水平均明顯升高，C 組和D組的IL-2/IL-10亦明顯升高，差異有統計學意義（均為P＜0.05）。與B組比較，C組和D組大鼠血清IL-2水平明顯升高，D組的IL-2/IL-10亦明顯升高，差異有統計學意義（均為P＜0.05）。與C組比較，D組大鼠血清IL-2水平明顯升高，D組的IL-2/IL-10亦明顯升高，差異有統計學意義（均為P＜0.05）。肝組織病理學檢查顯示，A組大鼠肝組織未見明顯排斥反應，排斥活動性指數（RAI）評分（1.8±0.7）分；B組RAI評分（3.1±1.3）分，屬輕度或不明確性排斥反應；C組RAI 評分（6.9±0.8）分，屬中～重度排斥反應；D組RAI 評分（8.8±0.5）分，屬重度排斥反應。各組間RAI 評分比較以及組間兩兩比較，差異均有統計學意義（均為P＜0.05）。A 組、B 組、C 組、D 組受體大鼠術後平均生存時間分彆為（119.3±1.9）d、（116.9±8.3）d、（53.4±6.1）d、（12.1±2.4）d，A組與B組的生存時間比較差異無統計學意義，餘各組兩兩比較差異均有統計學意義（均為P＜0.05）。結論4種組閤中，大鼠DA→Lewis模型排斥反應最劇烈，Lewis→BN次之，而Wistar→SD最輕，接近于免疫耐受。
목적：비교불동품계대서지간간이식배척반응특점。방법의거대서불동품계분성대조조SD→SD （A조），실험조Wistar→SD （B조）、Lewis→BN （C 조）이급 DA→Lewis （D조）4개조，채용Kamada쌍수투법건립대서원위간이식모형。비교각조수체대서술후10 d혈청간공능지표[병안산전안매（ALT）、총담홍소（TB）화백단백（Alb）]、혈청백세포개소（IL）-2화 IL-10수평、급성배척반응조직병이학분급화술후평균생존시간。결과여A、B조비교，C 조화D조적혈청ALT화TB명현승고， Alb 명현강저，차이균유통계학의의（균위 P＜0.05）。여 C 조비교， D조적TB명현승고，Alb명현강저，차이균유통계학의의（균위P＜0.05）。여A조비교，B、C、D조대서혈청IL-2화IL-10수평균명현승고，C 조화D조적IL-2/IL-10역명현승고，차이유통계학의의（균위P＜0.05）。여B조비교，C조화D조대서혈청IL-2수평명현승고，D조적IL-2/IL-10역명현승고，차이유통계학의의（균위P＜0.05）。여C조비교，D조대서혈청IL-2수평명현승고，D조적IL-2/IL-10역명현승고，차이유통계학의의（균위P＜0.05）。간조직병이학검사현시，A조대서간조직미견명현배척반응，배척활동성지수（RAI）평분（1.8±0.7）분；B조RAI평분（3.1±1.3）분，속경도혹불명학성배척반응；C조RAI 평분（6.9±0.8）분，속중～중도배척반응；D조RAI 평분（8.8±0.5）분，속중도배척반응。각조간RAI 평분비교이급조간량량비교，차이균유통계학의의（균위P＜0.05）。A 조、B 조、C 조、D 조수체대서술후평균생존시간분별위（119.3±1.9）d、（116.9±8.3）d、（53.4±6.1）d、（12.1±2.4）d，A조여B조적생존시간비교차이무통계학의의，여각조량량비교차이균유통계학의의（균위P＜0.05）。결론4충조합중，대서DA→Lewis모형배척반응최극렬，Lewis→BN차지，이Wistar→SD최경，접근우면역내수。
Objective Tocomparetherejectionoflivertransplantationindifferentstrains combinationofrats.Methods Fourtransplantationmodelsofvariousstraincombinationswereestablished including the control group SD (donor)to SD (recipient)(group A)and the experimental groups Wistar to SD (group B),Lewis to BN (group C)and DA to Lewis (group D). Orthotopic liver transplantation model of rats were constructed by Kamada's two cuff technique. And serum hepatic functions in 4 groups of recipient rats at 10th day after liver transplantation were compared,which was including alanine aminotransferase (ALT), total bilirubin (TB)and albumin (Alb). Meanwhile the serum levels of interleukin (IL)-2 and IL-10, rejection activity index (RAI)at 10th day after liver transplantation and the average survival time of recipient ratswereinvestigated.Results ComparedwithgroupAandB,theserumlevelsofALTandTBofgroupC and D increased obviously,while Alb decreased significantly. Compared with group C,TB of group D increased obviously,while Alb decreased remarkably. All of the differences were statistically significant (all in P <0.05). Compared with group A,the serum levels of IL-2 and IL-10 in group B,C and D all elevated significantly,and IL-2/IL-10 in group C and D increased remarkably too (all in P<0.05 ). Compared with group B,IL-2 in group C and D increased significantly,and IL- 2 /IL-10 in group D also elevated significantly. Compared with group C,both IL-2 and IL-2/IL-10 in group D increased significantly. All the differences were statistically significant (all in P<0.05 ). Hepatic histopathological examination showed that obvious rejection did not occur in the recipient rats of group A,which rejection activity index (RAI)was scored 1.8 ±0.7. The RAI score of Group B,C and D were 3.1 ±1.3,6.9 ±0.8 and 8.8 ±0.5,which belonged to mild or vague rejection,moderate to severe rejection and severe rejection respectively. All the RAI scores comparison among and between each group had statistical differences (all in P<0.05 ). The average survival time of recipient rats in group A,B,C and D were (119.3 ±1.9)d,(116.9 ±8.3)d,(53.4 ±6.1)d and (12.1 ±2.4)d respectively. There was no statistically significant difference between group A and B,while the comparisonbetweenanyothergroupwasstatisticallysignificant(allinP<0.05).Conclusions Infour different rats strains combination,the rejection is most serious in the model of DA (donor) to Lewis (recipient),moderate in Lewis to BN,mildest and near to tolerance in Wistar to SD.
