CAJ | 학술논문

目的：构建急性早幼粒细胞白血病细胞系HL-60、NB4的全反式维甲酸耐药株HL-60 R、NB4 R，并对其生物学性状进行鉴定。方法：以全反式维甲酸（ ATRA ）为诱导药物，采用浓度递增间歇诱导法构建HL-60、NB4细胞相应的ATRA耐药株。 CCK8法鉴定其ATRA耐药性，并检测耐药细胞株的生长曲线及多药耐药性。结果：成功构建了ATRA耐药细胞株HL-60 R、NB4 R，并发现与亲代细胞相比，其生长增殖速度减慢，群体倍增时间延长，且对其它多种化疗药物产生一定程度的交叉耐药。结论：成功构建的耐药细胞系HL-60R、NB4R为急性早幼粒细胞白血病多药耐药机制的进一步研究奠定了基础。
목적：구건급성조유립세포백혈병세포계HL-60、NB4적전반식유갑산내약주HL-60 R、NB4 R，병대기생물학성상진행감정。방법：이전반식유갑산（ ATRA ）위유도약물，채용농도체증간헐유도법구건HL-60、NB4세포상응적ATRA내약주。 CCK8법감정기ATRA내약성，병검측내약세포주적생장곡선급다약내약성。결과：성공구건료ATRA내약세포주HL-60 R、NB4 R，병발현여친대세포상비，기생장증식속도감만，군체배증시간연장，차대기타다충화료약물산생일정정도적교차내약。결론：성공구건적내약세포계HL-60R、NB4R위급성조유립세포백혈병다약내약궤제적진일보연구전정료기출。
Objective: To establish human acute promyelocytic leukemia ( APL ) ATRA-resistant cell lines and to explore their biological characteristics.Methods: Human APL ATRA-resistant cell lines HL-60 R and NB4 R were established by stepwise increasing concentrations of ATRA selection from the parental cell lines HL-60 and NB4.Drug sensitivity of resistant cells was detected by CCK8 assay,and the changes of biological characteristics were determined by light microscopy and cell counting.Results: Human APL ATRA-Resistant cell lines HL-60R and NB4R were developed with stable resistance to ATRA .Compared to parental cells, HL-60R and NB4R cells grew more slowly and had a longer doubling time .Besides,HL-60R and NB4R cells exhibited cross-resistance to etoposide , vincristine sulfate , cytarabine and doxorubicin .Conclusion:HL-60 R and NB4 R cells are reliable multi-drug resistant cell sublines of APL .The successful establishment of these cells has laid a solid foundation for further study of the multi-drug resistant mechanism of human APL induced by ATRA .