东南大学学报（医学版）
東南大學學報（醫學版）
동남대학학보（의학판）
JOURNAL OF SOUTHEAST UNIVERSITY(MEDICAL SCIENCE EDITION)
2014年
5期
555-560
,共6页
荧光探针%帕金森病%一氧化氮
熒光探針%帕金森病%一氧化氮
형광탐침%파금삼병%일양화담
fluorescence probe%Parkinson disease%nitric oxide
目的：研究帕金森病中一氧化氮（ NO）检测的新方法。方法：建立帕金森病的细胞与动物模型，检测小鼠相关行为学与生化指标的改变并通过新型探针MNIP-Cu荧光成像的方法检测NO浓度的变化。结果：帕金森病的细胞与动物模型中荧光探针MNIP-Cu检测到NO浓度显著升高，同时小鼠运动能力受到损伤、多巴胺合成减少；当NO合成受到抑制后，小鼠的运动能力和多巴胺的合成均显著恢复。荧光探针MNIP-Cu能够成功检测NO含量的变化。结论：新型荧光探针MNIP-Cu实现了对帕金森病体内外模型中NO的直接检测，为阐明NO在帕金森发病机制中的作用提供了新的研究手段。
目的：研究帕金森病中一氧化氮（ NO）檢測的新方法。方法：建立帕金森病的細胞與動物模型，檢測小鼠相關行為學與生化指標的改變併通過新型探針MNIP-Cu熒光成像的方法檢測NO濃度的變化。結果：帕金森病的細胞與動物模型中熒光探針MNIP-Cu檢測到NO濃度顯著升高，同時小鼠運動能力受到損傷、多巴胺閤成減少；噹NO閤成受到抑製後，小鼠的運動能力和多巴胺的閤成均顯著恢複。熒光探針MNIP-Cu能夠成功檢測NO含量的變化。結論：新型熒光探針MNIP-Cu實現瞭對帕金森病體內外模型中NO的直接檢測，為闡明NO在帕金森髮病機製中的作用提供瞭新的研究手段。
목적：연구파금삼병중일양화담（ NO）검측적신방법。방법：건립파금삼병적세포여동물모형，검측소서상관행위학여생화지표적개변병통과신형탐침MNIP-Cu형광성상적방법검측NO농도적변화。결과：파금삼병적세포여동물모형중형광탐침MNIP-Cu검측도NO농도현저승고，동시소서운동능력수도손상、다파알합성감소；당NO합성수도억제후，소서적운동능력화다파알적합성균현저회복。형광탐침MNIP-Cu능구성공검측NO함량적변화。결론：신형형광탐침MNIP-Cu실현료대파금삼병체내외모형중NO적직접검측，위천명NO재파금삼발병궤제중적작용제공료신적연구수단。
Objective:To develop a new method of nitric oxide detection in Parkinson disease .Methods:Using a Parkinson disease model in vitro and in vivo, we tested the behavior and biochemical changes of rat , and then detected the change of nitric oxide concentration through a novel fluorescent probe of MNIP-Cu.Results:The movement ability of rat was damaged with reduced dopamine synthesis in the Parkinson disease model .Consistently , the NO level was detected successfully by the MNIP-Cu fluorescence probe with significant increase .On the other hand, the rat's exercise capacity and dopamine production level was significantly restored after the inhibition of NO synthesis.Taking together, the NO production level can be detected successfully by the fluorescence probe of MNIP-Cu.Conclusion:We detect NO directly in Parkinson disease model in vitro and in vivo through novel fluorescent probe, which provides a new way to study the role of NO in the pathogenesis of the Parkinson disease.
