中国中西医结合肾病杂志
中國中西醫結閤腎病雜誌
중국중서의결합신병잡지
CHINESE JOURNAL OF INTEGRATED TRADITIONAL AND WESTERN NEPHROLOGY
2014年
9期
767-769
,共3页
韩利梅%方敬爱%孙艳艳%张晓东%刘文媛
韓利梅%方敬愛%孫豔豔%張曉東%劉文媛
한리매%방경애%손염염%장효동%류문원
糖尿病肾病%芡实%SOCS-3%IGF-1
糖尿病腎病%芡實%SOCS-3%IGF-1
당뇨병신병%검실%SOCS-3%IGF-1
Diabetic Nephropathy%Euryale ferox%SOCS-3%IGF-1
目的：探讨芡实对糖尿病肾病(diebetic nephrothy,DN)大鼠肾组织中细胞因子信号抑制因子-3(suppressor of cytokine signaling-3,SOCS-3)及胰岛素样生长因子-1(insulin like growth factor-1,IGF-1)表达的影响及可能机制。方法：采用单剂量腹腔注射链脲佐菌素( STZ,45 mg/kg)建立大鼠DN模型,将DN模型大鼠随机分为5组：DN组( DN组)、小剂量芡实组(EL,1.5 g·kg-1·d-1)、中剂量芡实组(EM,3.0 g·kg·-1d-1)、大剂量芡实组(EH,6.0 g·kg-1·d-1)及氯沙坦钾组( LP,30 mg·kg-1·d-1)；另设正常对照组( NC组),每组10只。分别治疗12周后测定各组大鼠生化指标；HE、Masson、PAS染色及电镜观察肾组织病理变化；免疫组化、Western blot法检测肾组织中SOCS-3与IGF-1表达情况。结果：12周末,与NC组相比,DN组大鼠24 h尿蛋白定量(24 h Upr)、尿素氮(BUN)及血肌酐(Scr)均明显升高(P<0.05),肾小球体积增大、系膜细胞增生、基质增多,肾小管扩张,肾组织中SOCS-3表达增加、IGF-1表达明显增加(P<0.05)；与DN组相比, LP组与EM组大鼠24 h Upr、BUN及Scr明显降低(P<0.05),病理改变减轻,肾组织SOCS-3表达明显增加、IGF-1表达明显下降(P<0.05)；LP组与EM组间降尿蛋白作用差异无统计学意义。结论：芡实可能通过上调SOCS-3表达,抑制IGF-1过表达进而减少蛋白尿,发挥肾脏保护作用。
目的：探討芡實對糖尿病腎病(diebetic nephrothy,DN)大鼠腎組織中細胞因子信號抑製因子-3(suppressor of cytokine signaling-3,SOCS-3)及胰島素樣生長因子-1(insulin like growth factor-1,IGF-1)錶達的影響及可能機製。方法：採用單劑量腹腔註射鏈脲佐菌素( STZ,45 mg/kg)建立大鼠DN模型,將DN模型大鼠隨機分為5組：DN組( DN組)、小劑量芡實組(EL,1.5 g·kg-1·d-1)、中劑量芡實組(EM,3.0 g·kg·-1d-1)、大劑量芡實組(EH,6.0 g·kg-1·d-1)及氯沙坦鉀組( LP,30 mg·kg-1·d-1)；另設正常對照組( NC組),每組10隻。分彆治療12週後測定各組大鼠生化指標；HE、Masson、PAS染色及電鏡觀察腎組織病理變化；免疫組化、Western blot法檢測腎組織中SOCS-3與IGF-1錶達情況。結果：12週末,與NC組相比,DN組大鼠24 h尿蛋白定量(24 h Upr)、尿素氮(BUN)及血肌酐(Scr)均明顯升高(P<0.05),腎小毬體積增大、繫膜細胞增生、基質增多,腎小管擴張,腎組織中SOCS-3錶達增加、IGF-1錶達明顯增加(P<0.05)；與DN組相比, LP組與EM組大鼠24 h Upr、BUN及Scr明顯降低(P<0.05),病理改變減輕,腎組織SOCS-3錶達明顯增加、IGF-1錶達明顯下降(P<0.05)；LP組與EM組間降尿蛋白作用差異無統計學意義。結論：芡實可能通過上調SOCS-3錶達,抑製IGF-1過錶達進而減少蛋白尿,髮揮腎髒保護作用。
목적：탐토검실대당뇨병신병(diebetic nephrothy,DN)대서신조직중세포인자신호억제인자-3(suppressor of cytokine signaling-3,SOCS-3)급이도소양생장인자-1(insulin like growth factor-1,IGF-1)표체적영향급가능궤제。방법：채용단제량복강주사련뇨좌균소( STZ,45 mg/kg)건립대서DN모형,장DN모형대서수궤분위5조：DN조( DN조)、소제량검실조(EL,1.5 g·kg-1·d-1)、중제량검실조(EM,3.0 g·kg·-1d-1)、대제량검실조(EH,6.0 g·kg-1·d-1)급록사탄갑조( LP,30 mg·kg-1·d-1)；령설정상대조조( NC조),매조10지。분별치료12주후측정각조대서생화지표；HE、Masson、PAS염색급전경관찰신조직병리변화；면역조화、Western blot법검측신조직중SOCS-3여IGF-1표체정황。결과：12주말,여NC조상비,DN조대서24 h뇨단백정량(24 h Upr)、뇨소담(BUN)급혈기항(Scr)균명현승고(P<0.05),신소구체적증대、계막세포증생、기질증다,신소관확장,신조직중SOCS-3표체증가、IGF-1표체명현증가(P<0.05)；여DN조상비, LP조여EM조대서24 h Upr、BUN급Scr명현강저(P<0.05),병리개변감경,신조직SOCS-3표체명현증가、IGF-1표체명현하강(P<0.05)；LP조여EM조간강뇨단백작용차이무통계학의의。결론：검실가능통과상조SOCS-3표체,억제IGF-1과표체진이감소단백뇨,발휘신장보호작용。
Objective:To investigate the effect and mechanism of euryale ferox on the expression of SOCS-3 and IGF-1 in renal tissues of diabetic nephropathy( DN) rats. Methods:The rat models of DN established by a single injection of streptozotocin were randomly divided into 5 groups, namely diabetic nephropathic model group ( group DN) , low-dose Euryale ferox group ( EL, 1.5 g·kg-1·d-1), medium -dose Euryale ferox group (EM, 3. 0 g·kg-1·d-1), high -dose Euryale ferox group (EH, 6. 0 g·kg-1·d-1) and losartan potassium group (LP, 30 mg·kg-1·d-1). With normal rats as the contro1, each group of 10. All the rats received daily gavage for 12 weeks, 24 h urinary protein (24 hUpr), blood urea nitrogen (BUN) and blood serum creati-nine ( Scr) were measured after the research. The renal pathological changes were observed by histochemistry staining and electron microscope. The expression of SOCS-3 and IGF-1 was detected by immunohistochemistry and western blot. Results:Compared with group NC, there were the glomerular hypertrophy, proliferation of mesangial cells, the accumulation of mesangial matrix and renal tu-bular expansion in group DN. 24 h Upr, BUN and Scr also increased significantly (P<0. 05). Meanwhile the expression of SOCS-3 and IGF-1 in renal tissues increased significantly (P<0. 05). Compared with group DN, the pathology changes were relieved, 24 h Upr, BUN, Scr, as well as the expression of IGF-1 decreased and SOCS-3 increased discinctly in group EM and group LP (P<0. 05). In addition, there was no significant difference in effect of reducing urinary protein in group EM and LP. Conclusion:Euryale ferox may reduce proteinuria and protect renal function by up-regulating the expression of SOCS-3, inhibiting the overex-pression of IGF-1.
