中国临床药理学杂志
中國臨床藥理學雜誌
중국림상약이학잡지
THE CHINESE JOURNAL OF CLINICAL PHARMACOLOGY
2014年
9期
807-809,815
,共4页
王伟%刘永锁%乔湜%李清艳
王偉%劉永鎖%喬湜%李清豔
왕위%류영쇄%교식%리청염
苯巴比妥%苯妥英钠%托吡酯%左乙拉西坦%超高效液相色谱法-质谱联用法
苯巴比妥%苯妥英鈉%託吡酯%左乙拉西坦%超高效液相色譜法-質譜聯用法
분파비타%분타영납%탁필지%좌을랍서탄%초고효액상색보법-질보련용법
phenobarbital%phenytoin sodium%topiramate%levertirace-tam%UPLC-MS/MS
目的：建立同时测定人血浆中苯巴比妥、苯妥英钠、托吡酯及左乙拉西坦浓度的方法。方法以磺胺甲噁唑（ SMZ）和吡格列酮为内标，血浆经甲醇直接沉淀后进样分析。色谱柱为 ACQUITY UPLC HSS PFP 柱，流动相为含0.1％甲酸的5 mmol· L-1乙酸铵水溶液-甲醇溶液，流速为0.2 mL· min-1，电喷雾离子源，用多反应监测，结合正负离子分段扫描分析。结果苯巴比妥、苯妥英钠、托吡酯及左乙拉西坦血药浓度分别在1.15～230.00（ r＝0.9973），0.10～20.20（ r＝0.9985），0.02～2.12（ r＝0.9965），0.10～20.40μg· mL-1（ r＝0.9963）内线性关系良好。日内、日间精密度（RSD）均＜15％；提取回收率均＞75％。结论该方法灵敏、快速、专属性强，可用于临床血药浓度测定及药代动力学研究。
目的：建立同時測定人血漿中苯巴比妥、苯妥英鈉、託吡酯及左乙拉西坦濃度的方法。方法以磺胺甲噁唑（ SMZ）和吡格列酮為內標，血漿經甲醇直接沉澱後進樣分析。色譜柱為 ACQUITY UPLC HSS PFP 柱，流動相為含0.1％甲痠的5 mmol· L-1乙痠銨水溶液-甲醇溶液，流速為0.2 mL· min-1，電噴霧離子源，用多反應鑑測，結閤正負離子分段掃描分析。結果苯巴比妥、苯妥英鈉、託吡酯及左乙拉西坦血藥濃度分彆在1.15～230.00（ r＝0.9973），0.10～20.20（ r＝0.9985），0.02～2.12（ r＝0.9965），0.10～20.40μg· mL-1（ r＝0.9963）內線性關繫良好。日內、日間精密度（RSD）均＜15％；提取迴收率均＞75％。結論該方法靈敏、快速、專屬性彊，可用于臨床血藥濃度測定及藥代動力學研究。
목적：건립동시측정인혈장중분파비타、분타영납、탁필지급좌을랍서탄농도적방법。방법이광알갑오서（ SMZ）화필격렬동위내표，혈장경갑순직접침정후진양분석。색보주위 ACQUITY UPLC HSS PFP 주，류동상위함0.1％갑산적5 mmol· L-1을산안수용액-갑순용액，류속위0.2 mL· min-1，전분무리자원，용다반응감측，결합정부리자분단소묘분석。결과분파비타、분타영납、탁필지급좌을랍서탄혈약농도분별재1.15～230.00（ r＝0.9973），0.10～20.20（ r＝0.9985），0.02～2.12（ r＝0.9965），0.10～20.40μg· mL-1（ r＝0.9963）내선성관계량호。일내、일간정밀도（RSD）균＜15％；제취회수솔균＞75％。결론해방법령민、쾌속、전속성강，가용우림상혈약농도측정급약대동역학연구。
Objective To develop the method for concentration determi-nation of phenobarbital , phenytoin sodium , topiramate and levertiracetam in human plasma.Methods UPLC -MS/MS was adopted to analyze plasma with protein precipitated by methanol.Sulfamethlazole (SMZ) and pioglitazone hydrochloride were as internal standard.Plasma samples were separated on ACQUITY UPLC HSS PFP column with aqueous solution (0.1%formic acid-5 mmol· L-1 ammonium acetate buffer )-methanol as mobile phase , and at a flow rate of 0.2 mL· min-1.Multiple reaction monitoring ( MRM) mode was performed combined with the ion switching technology for quantification in three sections.Results The liner calibra-tion curve of phenobarbital , phenytoin sodium , topiramate and levertirace-tam were obtained in the concentration range of 1.15 -230.00 ( r =0.997 3 ) , 0.10-20.20 ( r=0.998 5 ) , 0.02-2.12 ( r =0.996 5 ) and 0.10-20.40μg· mL-1(r=0.996 3), respectively.The lowest detection limit were 230.0, 20.20, 5.30,20.40 ng· mL-1 , respectively.The RSD of inter-day and intra -day were less than 15%.The relative recovery was more than 75%.Conclusion The method is accurate , sensitive and suitable for blood concentration monitoring and pharmacokinetic study of phenobarbital , phenytoin sodium , topiramate and levertiracetam.
