CAJ | 학술논문

目的：以睾酮为探针药物体外研究抗肿瘤中药注射液榄香烯、康莱特、鸦胆子油、艾迪对大鼠细胞色素P4503A4（CYP3A4）亚型酶活性的影响。方法用大鼠肝微粒体体外孵育法，用高效液相色谱法测定4种抗肿瘤中药注射液在孵育体系中6β-羟基睾酮（6-OHTS）生成量，反映其对CYP3A4酶活性的影响。结果与空白组相比，榄香烯对CYP3A4酶活性无明显影响；康莱特与艾迪对CYP3A4有诱导作用；鸦胆子油乳对CYP3A4有抑制作用，半数抑制率IC50值为0.78％。结论临床上在与经CYP3A4代谢的药物联合应用时，应警惕康莱特、艾迪及鸦胆子油乳潜在的药-药相互作用。
목적：이고동위탐침약물체외연구항종류중약주사액람향희、강래특、아담자유、애적대대서세포색소P4503A4（CYP3A4）아형매활성적영향。방법용대서간미립체체외부육법，용고효액상색보법측정4충항종류중약주사액재부육체계중6β-간기고동（6-OHTS）생성량，반영기대CYP3A4매활성적영향。결과여공백조상비，람향희대CYP3A4매활성무명현영향；강래특여애적대CYP3A4유유도작용；아담자유유대CYP3A4유억제작용，반수억제솔IC50치위0.78％。결론림상상재여경CYP3A4대사적약물연합응용시，응경척강래특、애적급아담자유유잠재적약-약상호작용。
Objective To investigate the influence of elemene , kang-laite, brucea jananica oil and Aidi injection on CYP 3A4 of liver micro-somes in vitro with testosterone as probe.Methods HPLC method was used to detect the production of 6β-hydroxytestosterone (6-OHTS) to study the effects of 4 anti-tumor traditional Chinese medicine injections on the activities of CYP 3 A4 enzymes in the microsomal incubation sys-tem.Results Compared with blank control group , elemene have no sig-nificant effects on CYP3A4; kanglaite, Aidi have a induction on CYP3A4;brucea jananica oil have a inhibition on CYP3A4, IC50 of bru-cea jananica oil on CYP3A4 was 0.78%.Conclusion Potential drug-drug interaction should be vigilant when kanglaite , aidi and brucea ja-nanica oil co -administered with the substrates which mainly undergo CYP3A4-mediated metabolism.