CAJ | 학술논문

目的：检测丙戊酸钠（ VPA）在动物体内对白血病细胞周期蛋白（ cyclins ）及细胞周期蛋白依赖性激酶（ CDKs）表达的影响。方法建立急性髓系白血病Kasumi-1细胞裸鼠移植瘤模型，分为对照组及VPA治疗组（腹腔注射VPA 500 mg· kg-1，2周），用RT-PCR法检测cyclinD1、cyclinE1和CDK4、CDK6、CDK2的mRNA的表达水平，用Western blot法检测cyclinD1蛋白的表达水平。结果与对照组相比，VPA治疗组cyclinD1、cyclinE1和CDK4、CDK6、CDK2 mRNA的表达明显下调（ P＜0.05）；cyclinD1蛋白的表达明显下降（ P＜0.01）。结论 VPA下调cyclinD1、cyclinE1、CDK4、CDK6和CDK2的表达，是VPA诱导肿瘤细胞周期阻滞的机制之一。
목적：검측병무산납（ VPA）재동물체내대백혈병세포주기단백（ cyclins ）급세포주기단백의뢰성격매（ CDKs）표체적영향。방법건립급성수계백혈병Kasumi-1세포라서이식류모형，분위대조조급VPA치료조（복강주사VPA 500 mg· kg-1，2주），용RT-PCR법검측cyclinD1、cyclinE1화CDK4、CDK6、CDK2적mRNA적표체수평，용Western blot법검측cyclinD1단백적표체수평。결과여대조조상비，VPA치료조cyclinD1、cyclinE1화CDK4、CDK6、CDK2 mRNA적표체명현하조（ P＜0.05）；cyclinD1단백적표체명현하강（ P＜0.01）。결론 VPA하조cyclinD1、cyclinE1、CDK4、CDK6화CDK2적표체，시VPA유도종류세포주기조체적궤제지일。
Objective To explore the molecular mechanism of valproic acid ( VPA) inducing tumor cell cycle arrest , by determining the expres-sion of cyclins and cyclin -dependent kinases ( CDKs).Methods Nude mice model with xenograft Kasumi -1 tumor was established by subcuta-neous inoculation of Kasumi -1 cells, and devided two groups: control group and VPA treatment group ( intraperitoneal injection of VPA 500 mg· kg-1 for 2 weeks).The expression levels of cyclinD1, cyclinE1, CDK4, CDK6, CDK2 mRNA were determined by semi -quantitative RT-PCR.The expression level of cyclinD 1 protein was determined by Western blot.Results Compared with the control group , VPA signifi-cantly reducted the expression of cyclinD 1, cyclinE1, CDK4, CDK6, CDK2 mRNA( P<0.05 );and reduced the expression of cyclinD 1 protein ( P<0.01 ).Conclusion The mechanism that VPA inductes cell cycle arrest is possibly due to downregulation of cyclin D 1, CDK4/6, cyclin E and CDK2 expression.