CAJ | 학술논문

目的：评价云南栘[木衣]叶萃取物的抗氧化活性，为云南栘[木衣]抗氧化功能食品、营养补充剂等的开发提供理论依据．方法：用系统溶剂萃取法将云南栘[木衣]叶的萃取物再分成石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物和水相4部分．用DPPH ·法和总还原力法评价云南栘[木衣]叶4个不同萃取物的抗氧化活性．用DPPH ·-HPLC-PDA和总还原力-HPLC-PDA柱前衍生法对云南栘[木衣]叶中的抗氧化成分进行了筛选．结果：云南栘[木衣]叶提取物不同极性分离物都具有良好的抗氧化活性，其中乙酸乙酯萃取物的萃取得率高达67.87％，DPPH ·半清除体积质量分数为0.079 m g/m L ，总还原力的芦丁质量分数为（0.19970±0.00031）g/g ，是云南栘[木衣]叶抗氧化活性的主要来源．云南栘[木衣]叶提取物中主要有9个抗氧化成分，主要集中于乙酸乙酯萃取物，其中4个为黄酮类化合物．结论：云南栘[木衣]叶是良好的天然抗氧化剂来源，具有抗氧化营养补充剂、功能食品的开发潜力，抗氧化成分筛选结果为云南栘[木衣]抗氧化功能的开发提供了方向．
목적：평개운남체[목의]협췌취물적항양화활성，위운남체[목의]항양화공능식품、영양보충제등적개발제공이론의거．방법：용계통용제췌취법장운남체[목의]협적췌취물재분성석유미췌취물、을산을지췌취물、정정순췌취물화수상4부분．용DPPH ·법화총환원역법평개운남체[목의]협4개불동췌취물적항양화활성．용DPPH ·-HPLC-PDA화총환원력-HPLC-PDA주전연생법대운남체[목의]협중적항양화성분진행료사선．결과：운남체[목의]협제취물불동겁성분리물도구유량호적항양화활성，기중을산을지췌취물적췌취득솔고체67.87％，DPPH ·반청제체적질량분수위0.079 m g/m L ，총환원력적호정질량분수위（0.19970±0.00031）g/g ，시운남체[목의]협항양화활성적주요래원．운남체[목의]협제취물중주요유9개항양화성분，주요집중우을산을지췌취물，기중4개위황동류화합물．결론：운남체[목의]협시량호적천연항양화제래원，구유항양화영양보충제、공능식품적개발잠력，항양화성분사선결과위운남체[목의]항양화공능적개발제공료방향．
The antioxidant activity of Docynia delavayiSchneid .leaf has been estimated in this paper .It provides theoretical basis for developing Docynia delavayiantioxidant nutritional supplement . Docynia delavayiSchneid .leaf extract has been separated by different polarity solvents :petroleum ether ,ethylace-tate ,n-butanol and water via systematic solvent extraction .Antioxidant activities of different polarity frac-tions have been evaluated by DPPH · assay and total reducing power assay .Its potential antioxidant com-pounds have also been screened by DPPH ·-HPLC-PDA assay and total reducing power-HPLC-PDA as-say .All polarity fractions of Docynia delavayiSchneid .leaf extract have exhibited high antioxidant activi-ty .The yield of ethylacetate fraction reached up to 67 .87% .The DPPH · SC50 value and total reducing power of ethylacetate fraction are 0 .079 mg/mL and (0 .19970 ± 0 .00031) g/g ,respectively .The antioxi-dant activity of Docynia delavayiSchneid .leaf mainly comes from ethylacetate fraction .9 antioxidants mainly concentrating in ethylacetate fraction are screened and 4 of them are flavones .It is concluded that Docynia delavayiSchneid .leaf is a good source of natural antioxidants and the result of antioxidants screening could be used to guide Docynia delavayiantioxidant nutritional supplement development .