热带农业科学
熱帶農業科學
열대농업과학
CHINESE JOURNAL OF TROPICAL AGRICULTURE
2014年
9期
33-36
,共4页
李静%王永%杨耀东%雷新涛%肖勇%夏薇
李靜%王永%楊耀東%雷新濤%肖勇%夏薇
리정%왕영%양요동%뢰신도%초용%하미
油棕%果实%RNA%提取
油棕%果實%RNA%提取
유종%과실%RNA%제취
Oil palm%Fruit%RNA%Extraction
获得高质量的 RNA 是开展油棕果实发育相关分子生物学基础研究的重要前提和保障。以油棕成熟果肉、胚乳、胚和胚芽4种组织为材料,分别采用 Invitrogen 公司的 Trizol 试剂和 MRIP 法提取总 RNA,并比较其提取效果。结果表明: MRIP 法提取的 RNA 质量较好,其中胚芽中的总 RNA 提取量高达2139.0 ng/μL,而胚乳中的含量相比较低,仅为1083.6 ng/μL; MRIP 法的总体提取效果优于 Trizol 试剂,包括 RNA 完整性、含量、纯度等,适用于油棕及棕榈科植物总 RNA 提取。
穫得高質量的 RNA 是開展油棕果實髮育相關分子生物學基礎研究的重要前提和保障。以油棕成熟果肉、胚乳、胚和胚芽4種組織為材料,分彆採用 Invitrogen 公司的 Trizol 試劑和 MRIP 法提取總 RNA,併比較其提取效果。結果錶明: MRIP 法提取的 RNA 質量較好,其中胚芽中的總 RNA 提取量高達2139.0 ng/μL,而胚乳中的含量相比較低,僅為1083.6 ng/μL; MRIP 法的總體提取效果優于 Trizol 試劑,包括 RNA 完整性、含量、純度等,適用于油棕及棕櫚科植物總 RNA 提取。
획득고질량적 RNA 시개전유종과실발육상관분자생물학기출연구적중요전제화보장。이유종성숙과육、배유、배화배아4충조직위재료,분별채용 Invitrogen 공사적 Trizol 시제화 MRIP 법제취총 RNA,병비교기제취효과。결과표명: MRIP 법제취적 RNA 질량교호,기중배아중적총 RNA 제취량고체2139.0 ng/μL,이배유중적함량상비교저,부위1083.6 ng/μL; MRIP 법적총체제취효과우우 Trizol 시제,포괄 RNA 완정성、함량、순도등,괄용우유종급종려과식물총 RNA 제취。
High quality of RNA is the important prerequisite and guarantee to basic research related to oil palm fruit development in molecular biology. RNA extracting effects of both Trizol reagents and MRIP (Methods for RNA Isolation from Palms) method developed by our laboratory previously were compared among four oil palm tissues, i.e. mature mesocarp, endosperm, embryo and germinated plumule. MRIP can get good quality RNA.The RNA content of plumule is as high as 2 139.0 ng/μL, while that of endosperm is only 1 083.6 ng/μL. MRIP is generally better than Trizol method in respect of the integrality, content and purity of extracted RNA, which Suitable for oil palm and palm plants RNA extraction.