热带农业科学
熱帶農業科學
열대농업과학
CHINESE JOURNAL OF TROPICAL AGRICULTURE
2014年
9期
30-32
,共3页
符运柳%徐立%李志英%黄碧兰%李克烈
符運柳%徐立%李誌英%黃碧蘭%李剋烈
부운류%서립%리지영%황벽란%리극렬
蜻蜓凤梨%叶片%组织培养%植株再生
蜻蜓鳳梨%葉片%組織培養%植株再生
청정봉리%협편%조직배양%식주재생
Aechmea fasciata%leaf%tissue culture%plant regeneration
以蜻蜓凤梨试管苗叶片为外植体进行离体再生研究。结果表明,叶片诱导直接分化不定芽的最佳培养基是 MS+BA 5.0 mg/L+IBA 1.5 mg/L,分化率高达80%。增殖培养基为 MS+BA 0.5 mg/L+NAA 0.2 mg/L。壮苗培养基为 MS+NAA 2.0 mg/L。生根培养基为 MS+NAA 2.0 mg/L+AC 0.5 g/L,生根率达100%。
以蜻蜓鳳梨試管苗葉片為外植體進行離體再生研究。結果錶明,葉片誘導直接分化不定芽的最佳培養基是 MS+BA 5.0 mg/L+IBA 1.5 mg/L,分化率高達80%。增殖培養基為 MS+BA 0.5 mg/L+NAA 0.2 mg/L。壯苗培養基為 MS+NAA 2.0 mg/L。生根培養基為 MS+NAA 2.0 mg/L+AC 0.5 g/L,生根率達100%。
이청정봉리시관묘협편위외식체진행리체재생연구。결과표명,협편유도직접분화불정아적최가배양기시 MS+BA 5.0 mg/L+IBA 1.5 mg/L,분화솔고체80%。증식배양기위 MS+BA 0.5 mg/L+NAA 0.2 mg/L。장묘배양기위 MS+NAA 2.0 mg/L。생근배양기위 MS+NAA 2.0 mg/L+AC 0.5 g/L,생근솔체100%。
The regeneration system of Aechmea fasciata in vitro was studied with leaves as explants. The results showed the optimal medium for shoots induction was MS+BA 5.0 mg/L+IBA 1.5 mg/L with the induction frequency of 80%. The shoot multiplying medium was MS+BA 0.5 mg/L+NAA 0.2 mg/L. The elongation medium was MS+NAA 2.0 mg/L. The rooting medium was MS+NAA 2.0 mg/L+AC 0.5 g/L. The rooting frequency was 100%.