临床儿科杂志
臨床兒科雜誌
림상인과잡지
2014年
9期
825-828
,共4页
新生儿%感染%T淋巴细胞亚群%白介素4%白介素17%γ-干扰素
新生兒%感染%T淋巴細胞亞群%白介素4%白介素17%γ-榦擾素
신생인%감염%T림파세포아군%백개소4%백개소17%γ-간우소
newborn%infection%T lymphocyte subsets%interleulkin-4%interleukin-17%interferon-γ
目的：探讨新生儿感染不同病原体后体内相关免疫细胞及其细胞因子的变化。方法入选20例新生儿细菌性肺炎患儿(细菌组)，15例新生儿轮状病毒腹泻患儿(病毒组)，20例新生儿高胆红素血症患儿(对照组)，采用流式细胞仪检测外周血CD4+T淋巴细胞因子、淋巴细胞亚群。结果细菌组、病毒组和对照组间，IL-4水平的差异有统计学意义(F=3.39，P=0.041)，IFN-γ、IL-17水平在三组间的差异均无统计学意义(F=0.28、1.24，P均>0.05)；经两两比较发现，细菌组IL-4水平高于病毒组和对照组，差异有统计学意义(P均<0.05)。除CD8+细胞外，CD3+、CD4+、CD19+、NK细胞阳性率以及CD4+/CD8+，在细菌组、病毒组和对照组三组间的差异均有统计学意义(F=3.30~26.69，P均<0.05)。经两两比较发现，CD3+和CD4+细胞阳性率以及CD4+/CD8+，细菌组和病毒组均低于对照组；细菌组和病毒组CD19+细胞阳性率均高于对照组，差异均有统计学意义(P均<0.05)。病毒组NK细胞阳性率低于对照组和细菌组，差异均有统计学意义(P<0.05)。结论不同病原体感染后体内相关细胞因子水平变化不同，免疫功能紊乱，体内存在Th1/Th2失衡，以Th2型占主导地位。T淋巴细胞亚群水平较低，提示机体感染后细胞免疫功能减低，也可能是新生儿易感及感染后病情隐匿、病程长的原因之一。
目的：探討新生兒感染不同病原體後體內相關免疫細胞及其細胞因子的變化。方法入選20例新生兒細菌性肺炎患兒(細菌組)，15例新生兒輪狀病毒腹瀉患兒(病毒組)，20例新生兒高膽紅素血癥患兒(對照組)，採用流式細胞儀檢測外週血CD4+T淋巴細胞因子、淋巴細胞亞群。結果細菌組、病毒組和對照組間，IL-4水平的差異有統計學意義(F=3.39，P=0.041)，IFN-γ、IL-17水平在三組間的差異均無統計學意義(F=0.28、1.24，P均>0.05)；經兩兩比較髮現，細菌組IL-4水平高于病毒組和對照組，差異有統計學意義(P均<0.05)。除CD8+細胞外，CD3+、CD4+、CD19+、NK細胞暘性率以及CD4+/CD8+，在細菌組、病毒組和對照組三組間的差異均有統計學意義(F=3.30~26.69，P均<0.05)。經兩兩比較髮現，CD3+和CD4+細胞暘性率以及CD4+/CD8+，細菌組和病毒組均低于對照組；細菌組和病毒組CD19+細胞暘性率均高于對照組，差異均有統計學意義(P均<0.05)。病毒組NK細胞暘性率低于對照組和細菌組，差異均有統計學意義(P<0.05)。結論不同病原體感染後體內相關細胞因子水平變化不同，免疫功能紊亂，體內存在Th1/Th2失衡，以Th2型佔主導地位。T淋巴細胞亞群水平較低，提示機體感染後細胞免疫功能減低，也可能是新生兒易感及感染後病情隱匿、病程長的原因之一。
목적：탐토신생인감염불동병원체후체내상관면역세포급기세포인자적변화。방법입선20례신생인세균성폐염환인(세균조)，15례신생인륜상병독복사환인(병독조)，20례신생인고담홍소혈증환인(대조조)，채용류식세포의검측외주혈CD4+T림파세포인자、림파세포아군。결과세균조、병독조화대조조간，IL-4수평적차이유통계학의의(F=3.39，P=0.041)，IFN-γ、IL-17수평재삼조간적차이균무통계학의의(F=0.28、1.24，P균>0.05)；경량량비교발현，세균조IL-4수평고우병독조화대조조，차이유통계학의의(P균<0.05)。제CD8+세포외，CD3+、CD4+、CD19+、NK세포양성솔이급CD4+/CD8+，재세균조、병독조화대조조삼조간적차이균유통계학의의(F=3.30~26.69，P균<0.05)。경량량비교발현，CD3+화CD4+세포양성솔이급CD4+/CD8+，세균조화병독조균저우대조조；세균조화병독조CD19+세포양성솔균고우대조조，차이균유통계학의의(P균<0.05)。병독조NK세포양성솔저우대조조화세균조，차이균유통계학의의(P<0.05)。결론불동병원체감염후체내상관세포인자수평변화불동，면역공능문란，체내존재Th1/Th2실형，이Th2형점주도지위。T림파세포아군수평교저，제시궤체감염후세포면역공능감저，야가능시신생인역감급감염후병정은닉、병정장적원인지일。
Objective To study the T Lymphocyte Subsets and the cytokines in the newborns with infectious diseases. Methods Twenty cases of neonatal bacterial pneumonia (bacterial group), 15 cases of rotavirus enteritis (virus group) and 20 newborns with jaundice (control group) were recruited in this study. The peripheral CD4+T Cells and lymphocyte subsets were assessed by flow cytometry. Results The IL-4 level was significantly different among different groups (F=3.39, P=0.041). The levels of IL-17 and IFN-γdid not differ signiifcantly among different groups (F=0.28 and 1.24 respectively, P>0.05). The IL-4 level was higher in bacterial group than that in virus group and control group (P<0.05). The percentages of CD3+, CD4+, CD19+and NK cells and the ratio of CD4+/CD8+ had significant difference among different groups (F=3.30-26.69, P<0.05). The percentages of CD3+and CD4+cells and the ratio of CD4+/CD8+were lower in bacterial group and virus group than those in control group (P<0.05). The percentage of CD19+cells was higher in bacterial group and virus group than that in control group (P<0.05). The percentage of NK cells was lower in virus group than that in bacterial group and control group (P<0.05). Conclusions The pattern of cytokines level is different in newborns with infection caused by different pathogens. Newborns with infectious diseases have immune dysfunction and Th2-dominated imbalance. The low percentages of T lymphocyte subsets indicate the depressed cellular immunity after infection, which may result in atypical symptom and prolonged disease course.
