CAJ | 학술논문

为研究黄芪多糖对猪瘟活疫苗的佐剂作用，分别用0.5％黄芪多糖、疫苗专用稀释液、生理盐水（对照）稀释猪瘟耐热保护剂活疫苗免疫猪，测定免疫后不同时间段内血清中 IgG 及其亚型IgG1、IgG2、IgG3、IgG4和细胞因子IL 2、IL 6的含量。结果显示，与对照组相比，黄芪多糖作为疫苗稀释液可以显著提高血清中IgG及其亚型和细胞因子IL 2、IL 6的含量。与疫苗专用稀释液组相比，在免疫后第7、14、28天，黄芪多糖组IgG含量均提高，分别提高1.6％（P＞0.05）、6.4％（P＜0.05）、7.1％（P＜0.05），IL 2含量分别提高12.1％（P＜0.05）、2.9％（P＞0.05）、15.3％（P＜0.05），IL 6含量差异均不显著。表明，黄芪多糖作为疫苗稀释液对猪瘟活疫苗具有良好的佐剂作用。
위연구황기다당대저온활역묘적좌제작용，분별용0.5％황기다당、역묘전용희석액、생리염수（대조）희석저온내열보호제활역묘면역저，측정면역후불동시간단내혈청중 IgG 급기아형IgG1、IgG2、IgG3、IgG4화세포인자IL 2、IL 6적함량。결과현시，여대조조상비，황기다당작위역묘희석액가이현저제고혈청중IgG급기아형화세포인자IL 2、IL 6적함량。여역묘전용희석액조상비，재면역후제7、14、28천，황기다당조IgG함량균제고，분별제고1.6％（P＞0.05）、6.4％（P＜0.05）、7.1％（P＜0.05），IL 2함량분별제고12.1％（P＜0.05）、2.9％（P＞0.05）、15.3％（P＜0.05），IL 6함량차이균불현저。표명，황기다당작위역묘희석액대저온활역묘구유량호적좌제작용。
The present study aimed to investigate the effects of Astragalus polysaccharide(APS) on immune response of activated vaccine of swine fever,separately using 0.5% of the APS and swine fever vaccine special diluent dilution for activated vaccine of swine fever.After vaccination, content of specific IgG,subclasses IgG1,IgG2,IgG3,IgG4 and IL-2,IL-6 in serum were measured in different time period.The results showed that compared with special diluent vaccine group, APS as a vaccine diluent could significantly improve IgG and its subtype,IL-2 and IL-6 content in serum.On days of 7,14,28 after immunization,IgG levels of APS group were improved, respectively increased by 1.6%(P>0.05 ),6.4%(P<0.05 ),7.1%(P<0.05 ),IL-2 content increased by 12.1%(P<0.05),2.9%(P>0.05),15.3%(P<0.05),but no significant difference was observed in the content of IL-6.It could be inferred that APS had good adjuvant effect on vaccine inoculation of swine fever used as immune diluent.