CAJ | 학술논문

以铁皮石斛原球茎为繁殖体，初步建立适合铁皮石斛的人工种子制备工艺，为铁皮石斛的高效繁育提供一条新技术途径。结果表明，经过优化组合，以 MS＋100 g/L 土豆汁＋0.5 mg/L NAA＋30 g/L海藻酸钠＋30 g/L蔗糖＋1.0 g/L保水剂＋1.0 g/L木薯淀粉＋0.1 g/L 活性炭（AC）为人工胚乳，在2％ CaC12中反应15 min后获得的铁皮石斛人工种子呈规则的圆球形、富有弹性，培养15 d后失水率为11.17％，培养60 d后萌发率达到92.86％。此配方为制备铁皮石斛人工种子的最优组合。
이철피석곡원구경위번식체，초보건립괄합철피석곡적인공충자제비공예，위철피석곡적고효번육제공일조신기술도경。결과표명，경과우화조합，이 MS＋100 g/L 토두즙＋0.5 mg/L NAA＋30 g/L해조산납＋30 g/L자당＋1.0 g/L보수제＋1.0 g/L목서정분＋0.1 g/L 활성탄（AC）위인공배유，재2％ CaC12중반응15 min후획득적철피석곡인공충자정규칙적원구형、부유탄성，배양15 d후실수솔위11.17％，배양60 d후맹발솔체도92.86％。차배방위제비철피석곡인공충자적최우조합。
In order to establish an efficient procedure for D.officinale artificial seeds production, the preparation technology for D.officinale artificial seeds was established using its protocorms as propagules.The results showed that when MS+100 g/L potato juice+0.5 mg/L NAA+30 g/L sodium alginate+30 g/L sucrose+1.0 g/L water retention agent+1.0 g/L cassava starch+0.1 g/L AC was used as artificial endosperm,the spherical and elastic artificial seeds were achieved after dropping the artificial endosperm into the solution of 2% CaCl2 for 1 5 min,and the rate of water loss was 1 1.1 7% in artificial seeds after storage for 1 5 d,the germination rate of the artificial seeds was 92.86% after storage for 60 d.The above formula was the best combination of various ingredients for D.officinale artificial seeds production.