CAJ | 학술논문

为了筛选黄瓜枯萎病拮抗放线菌，从蔬菜温室大棚及附近土壤中分离了59株菌落形态有差异的放线菌，通过平板对峙筛选试验，获得2株对黄瓜枯萎病菌抑菌率在60％以上的放线菌，分别是S21和S24，其中 S24的抑菌率达到74％。根据形态特征、生理生化特性和分子分类测定结果，初步将S24鉴定为黄麻链霉菌（Streptomyces corchorusii）。选用7种常用的放线菌发酵培养基对S24进行发酵，7 d后测定发现，5号培养基发酵上清液对黄瓜枯萎病菌的抑制活性最强，抑菌率达到59.44％，其细胞破碎液对黄瓜枯萎病菌的抑菌率为39.76％。通过比较发现，75 mL为放线菌S24的最适发酵装液量（250 mL三角瓶）。采用正交试验优化后的培养基进行发酵，获得的上清液及细胞破碎液对黄瓜枯萎病菌的抑菌率分别达到76.14％、52.27％，较优化前分别提高了14.64、10.11个百分点。
위료사선황과고위병길항방선균，종소채온실대붕급부근토양중분리료59주균락형태유차이적방선균，통과평판대치사선시험，획득2주대황과고위병균억균솔재60％이상적방선균，분별시S21화S24，기중 S24적억균솔체도74％。근거형태특정、생리생화특성화분자분류측정결과，초보장S24감정위황마련매균（Streptomyces corchorusii）。선용7충상용적방선균발효배양기대S24진행발효，7 d후측정발현，5호배양기발효상청액대황과고위병균적억제활성최강，억균솔체도59.44％，기세포파쇄액대황과고위병균적억균솔위39.76％。통과비교발현，75 mL위방선균S24적최괄발효장액량（250 mL삼각병）。채용정교시험우화후적배양기진행발효，획득적상청액급세포파쇄액대황과고위병균적억균솔분별체도76.14％、52.27％，교우화전분별제고료14.64、10.11개백분점。
To screen antagonistic actinomycetes against Fusarisum oxysporum f.sp.cucumebrium (F.o.c),fifty-nine actinomycetes showing difference in morphology were isolated from greenhouse and surrounding soils through plate cultivation,and among them two strains,S21 and S24,with the inhibition rate over 60%,were acquired.The inhibition rate of S24 to F.o.c reached 74%. Combined with morphological characteristics,physiological and biochemical properties and molecular determination results,S24 was preliminarily identified as Streptomyces corchorusii. Seven commonly used fermentation media were selected for screening,and the result showed that the supernatant of No.5 medium after 7-day fermentation displayed strongest inhibition activity to F.o.c with the inhibition rate reaching 5 9.44%,meanwhile the inhibition rate of the debris reached 39.76%.The optimum volume of fermentation medium was 75 mL in 250 mL flask for S24.Through optimization of No.5 medium by orthogonal design,the optimum fermentation medium composition of S24 was found,and the inhibition rates of the fermentation supernatant and debris of S24 to F.o.c reached 76.14% and 52.27%,increased by 14.64,10.11 percentage points,respectively.