CAJ | 학술논문

为了获得具有高效解磷活性和应用潜力的根际促生菌，从河南省信阳黄棕壤茶区采集茶树根际土壤样品，采用选择性培养基，以稀释培养法筛选具有解磷能力的细菌。结果表明，共筛选获得具有较强解磷能力的根际细菌36株，其中，解无机磷细菌23株，解有机磷细菌13株；解磷细菌主要分布于芽孢杆菌属、类芽孢杆菌属、伯克氏菌属、肠杆菌属、不动杆菌属和假单胞菌属，其中，芽孢杆菌和类芽孢杆菌为优势种群。解磷特性和活性测定结果表明，蜡样芽孢杆菌、类芽孢杆菌和伯克氏菌兼具解无机磷和有机磷的作用，而假单胞菌仅具有解有机磷的作用；23株解无机磷细菌中，18株的解磷活性大于100μg/mL，13株解有机磷细菌的解磷活性均大于40μg/mL。
위료획득구유고효해린활성화응용잠력적근제촉생균，종하남성신양황종양다구채집다수근제토양양품，채용선택성배양기，이희석배양법사선구유해린능력적세균。결과표명，공사선획득구유교강해린능력적근제세균36주，기중，해무궤린세균23주，해유궤린세균13주；해린세균주요분포우아포간균속、류아포간균속、백극씨균속、장간균속、불동간균속화가단포균속，기중，아포간균화류아포간균위우세충군。해린특성화활성측정결과표명，사양아포간균、류아포간균화백극씨균겸구해무궤린화유궤린적작용，이가단포균부구유해유궤린적작용；23주해무궤린세균중，18주적해린활성대우100μg/mL，13주해유궤린세균적해린활성균대우40μg/mL。
In order to screen the strains with potential applications in microbial fertilizer,phosphate-solubilizing microorganisms were screened from rhizosphere soil of tea plants grown in brown-yellow soil of Xinyang area in Henan province,using dilution-culture method on selective media.The results showed that a total of 36 phosphate-solubilizing bacterial strains(PSBs)were obtained.Among the 36 PSBs,23 strains could solubilize inorganic phosphate,and 1 3 strains could solubilize organic phosphate.The 3 6 PSBs mainly distributed in Bacillus,Paenibacillus,Burkholdeia,Enterobacter,Acinetobacter and Pseudomonas,in which,Bacillus spp.and Paenibacillus spp.were the dominant population in rhizosphere soil of tea plants.Phosphate-solubilizing ability of 3 6 PSBs were assayed,and the results indicated that strains of Bacillus cereus,Paenibacillus spp.and Burkholdeia spp.could solubilize both inorganic phosphate and organic phosphate,but strains of Pseudomonas spp.could only solubilize organic phosphate.Among 2 3 inorganic PSBs,the inorganic phosphate-solubilizng activities of 1 8 strains were more than 100μg/mL,and the organic phosphate-solubilizng activities of 13 strains were more than 4 0μg/mL.