农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2014年
9期
1457-1465
,共9页
王红春%李俊%吕波%朱旭东%娄远来%董立尧
王紅春%李俊%呂波%硃旭東%婁遠來%董立堯
왕홍춘%리준%려파%주욱동%루원래%동립요
早熟禾%精噁唑禾草灵%耐性%乙酰辅酶A羧化酶
早熟禾%精噁唑禾草靈%耐性%乙酰輔酶A羧化酶
조숙화%정오서화초령%내성%을선보매A최화매
Poa annua L.%Fenoxaprop-P-ethyl%Tolerance%Acetyl-CoA carboxylase
早熟禾对精噁唑禾草灵、精喹禾灵、高效氟吡甲禾灵、炔草酸、精吡氟禾草灵、氰氟草酯、烯禾啶和苯草酮具有耐药性,而对烯草酮和吡喃草酮敏感。早熟禾生物型的乙酰辅酶 A羧化酶(ACCase)对精噁唑禾草灵的活性显著高于敏感杂草日本看麦娘,5个早熟禾生物型的 IC50值为敏感杂草日本看麦娘 IC50值的10.46~11.98倍;早熟禾 ACCase氨基酸序列1781位点存在亮氨酸/异亮氨酸的多态性现象,而亮氨酸的存在是其它禾本科杂草对ACCase抑制剂除草剂产生抗性的主要原因之一;5个早熟禾生物型的 ACCase基因表达量为日本看麦娘的4.67~7.37倍。早熟禾与日本看麦娘的 ACCase活性、基因序列及基因表达量差异可能是导致早熟禾对精噁唑禾草灵具有耐药性的靶标酶机理。
早熟禾對精噁唑禾草靈、精喹禾靈、高效氟吡甲禾靈、炔草痠、精吡氟禾草靈、氰氟草酯、烯禾啶和苯草酮具有耐藥性,而對烯草酮和吡喃草酮敏感。早熟禾生物型的乙酰輔酶 A羧化酶(ACCase)對精噁唑禾草靈的活性顯著高于敏感雜草日本看麥孃,5箇早熟禾生物型的 IC50值為敏感雜草日本看麥孃 IC50值的10.46~11.98倍;早熟禾 ACCase氨基痠序列1781位點存在亮氨痠/異亮氨痠的多態性現象,而亮氨痠的存在是其它禾本科雜草對ACCase抑製劑除草劑產生抗性的主要原因之一;5箇早熟禾生物型的 ACCase基因錶達量為日本看麥孃的4.67~7.37倍。早熟禾與日本看麥孃的 ACCase活性、基因序列及基因錶達量差異可能是導緻早熟禾對精噁唑禾草靈具有耐藥性的靶標酶機理。
조숙화대정오서화초령、정규화령、고효불필갑화령、결초산、정필불화초령、청불초지、희화정화분초동구유내약성,이대희초동화필남초동민감。조숙화생물형적을선보매 A최화매(ACCase)대정오서화초령적활성현저고우민감잡초일본간맥낭,5개조숙화생물형적 IC50치위민감잡초일본간맥낭 IC50치적10.46~11.98배;조숙화 ACCase안기산서렬1781위점존재량안산/이량안산적다태성현상,이량안산적존재시기타화본과잡초대ACCase억제제제초제산생항성적주요원인지일;5개조숙화생물형적 ACCase기인표체량위일본간맥낭적4.67~7.37배。조숙화여일본간맥낭적 ACCase활성、기인서렬급기인표체량차이가능시도치조숙화대정오서화초령구유내약성적파표매궤리。
Annual bluegrass (Poa annua L.) was found to be tolerant to fenoxaprop-P-ethyl as wel as quizalofop-P-ethyl, haloxyfop-R-methyl, clodinafop-propargyl, fluaz-ifop-P-butyl, cyhalofop-butyl, sethoxydim and tralkoxydim, whereas it was sensitive to clethodim and tepraloxydim. The acetyl-CoA carboxylase (ACCase) IC50 values of five P. annua biotypes were 10.46 to 11.98-fold higher than the susceptible Japanese foxtail (Alopecurus japonicus Steud.). The presence of the polymorphic lle and Leu at 1 781, which the presence of Leu at 1 781 had been reported to be in-volved in the resistance of grass weeds to ACCase inhibitors, was subsequently i-dentified in the ACCase of P. annua. Furthermore, the expression level of gene that encoding P. annua ACCase was found to be approximately 4.67 to 7.37-fold higher than A. japonicus, possibly explaining the P. annua target site tolerance to fenoxaprop-P-ethyl.