CAJ | 학술논문

目的通过510例股骨头标本HE制片结果的分析,比较常规脱水与超声波快速脱水的效果.方法股骨头标本用锯锯成2cm×2cm×0.3cm的薄片二份,用30％的甲酸脱钙液进行脱钙16～18小时,分别用常规脱水的方法及超声波快速脱水的方法脱水处理后,经石蜡包埋、切片、染色制成HE切片,观察染色后的组织结构的清晰度及完整性.结果股骨头标本经二种脱水方法处理后制成的HE切片,阅片比较发现超声波快速脱水法优良率略高于常规脱水法,两种方法比较,P＞0.05,差异无统计学意义.但超声波组织处理仪能明显缩短病理制片工作时间.结论骨组织脱钙后采用超声波快速脱水的方法可以代替常规脱水,HE切片效果良好,缩短了病理检验报告时间,更好地满足了临床工作的需求.
목적 통과510례고골두표본HE제편결과적분석,비교상규탈수여초성파쾌속탈수적효과.방법 고골두표본용거거성2cm×2cm×0.3cm적박편이빈,용30％적갑산탈개액진행탈개16～18소시,분별용상규탈수적방법급초성파쾌속탈수적방법탈수처리후,경석사포매、절편、염색제성HE절편,관찰염색후적조직결구적청석도급완정성.결과 고골두표본경이충탈수방법처리후제성적HE절편,열편비교발현초성파쾌속탈수법우량솔략고우상규탈수법,량충방법비교,P＞0.05,차이무통계학의의.단초성파조직처리의능명현축단병리제편공작시간.결론 골조직탈개후채용초성파쾌속탈수적방법가이대체상규탈수,HE절편효과량호,축단료병리검험보고시간,경호지만족료림상공작적수구.
Objective To compare the effect of conventional dehydration with that of rapid ultrasonic dehydration by analyzing the results of tissue slices of femoral head specimens with HE stainning from 510 patients.Methods The femoral head specimens were sawn into two pieces of slice in 2cm × 2cm × 0.3cm;30％ formic acid-decalcification solution was used to make the speimens decalcified in 16-18 hours;then the pieces of slice were treated by conventional dehydration or ultrasonic rapid dehydration.After being paraffin-embedded,sliced??,and stained,the clarity and completeness of the tinct organizational structure of the specimens with HE stainning were observed.Results The effect of rapid ultrasonic dehydration was slightly better than conventional dehydration for the tissue slices of femoral head specimens with HE stainning.But there was no statistical significance between the two methods(P＞0.05).Conclusions After decalcification of bone tissues,rapid ultrasonic dehydration can be used to replace conventional dehydration for obtaining better tissue slices with HE staining.Rapid ultrasonic dehydration shortens the time of pathological results,which can satisfy the demand for clinical work.