CAJ | 학술논문

目的探讨七氟醚后处理对肢体缺血再灌注诱发大鼠肾损伤的影响.方法健康成年雄性SD大鼠24只,体重200～ 250 g,采用随机数字表法,将大鼠随机分为3组(n=8):假手术组(S组)、肢体缺血再灌注组(I/R组)和七氟醚后处理组(Spo组).I/R组和Spo组制备肢体缺血再灌注模型,双后肢缺血4h、再灌注6h,S组仅作假手术对照.Spo组再灌注前吸入2.5％七氟醚,持续6h.于再灌注6h时下腔静脉取血测定血清尿素氮(BUN)和肌酐(Cr)浓度；采用化学比色法测定肾组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量；采用酶联免疫吸附法测定TNF-α和IL-6的含量；光镜下观察肾组织病理学结果.结果与S组比较,I/R组及Spo组BUN、Cr、MDA、TNF-α和IL-6水平升高,SOD活性降低(P＜0.05),肾组织损伤明显；与I/R组比较,Spo组BUN、Cr、MDA、TNF-α和IL-6水平降低,SOD活性升高(P＜0.05),肾组织损伤减轻.结论七氟醚后处理可减轻大鼠肢体缺血再灌注诱发的肾损伤,其机制可能与减少氧自由基释放和抑制炎症反应有关.
목적 탐토칠불미후처리대지체결혈재관주유발대서신손상적영향.방법 건강성년웅성SD대서24지,체중200～ 250 g,채용수궤수자표법,장대서수궤분위3조(n=8):가수술조(S조)、지체결혈재관주조(I/R조)화칠불미후처리조(Spo조).I/R조화Spo조제비지체결혈재관주모형,쌍후지결혈4h、재관주6h,S조부작가수술대조.Spo조재관주전흡입2.5％칠불미,지속6h.우재관주6h시하강정맥취혈측정혈청뇨소담(BUN)화기항(Cr)농도；채용화학비색법측정신조직초양화물기화매(SOD)활성화병이철(MDA)함량；채용매련면역흡부법측정TNF-α화IL-6적함량；광경하관찰신조직병이학결과.결과 여S조비교,I/R조급Spo조BUN、Cr、MDA、TNF-α화IL-6수평승고,SOD활성강저(P＜0.05),신조직손상명현；여I/R조비교,Spo조BUN、Cr、MDA、TNF-α화IL-6수평강저,SOD활성승고(P＜0.05),신조직손상감경.결론 칠불미후처리가감경대서지체결혈재관주유발적신손상,기궤제가능여감소양자유기석방화억제염증반응유관.
Objective To investigate the effect of sevoflurane postconditioning on the renal injury induced by hind limb ischemia-reperfusion (I/R) in rats.Methods Twenty-four healthy male Sprague-Dawley rats,weighing 200-250 g,were randomly assigned into 3 groups (n =8 each):sham operation group (group S),group I/R and sevoflurane group (group Spo).Limb ischemia was induced by occlusion of bilateral hind limbs for 4 h followed by 6 h reperfusion.In group Spo,sevoflurane was inhaled for 6 h at the end-tidal concentration of 2.5 ％ before reperfusion,while pure oxygen was inhaled instead of sevoflurane in groups S and I/R.Blood samples were taken from the inferior vena cava at 6 h of reperfusion to determine the serum blood urea nitrogen (BUN) and creatinine (Cr) concentrations.The rats were then sacrificed and the kidney was removed for determination of superoxide dismutase (SOD) activity and contents of malondialdehyde (MDA),tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and for microscopic examination.Results The levels of BUN,Cr,MDA,TNF-α and IL-6 were significantly higher,the SOD content was significantly lower (P ＜ 0.05),and the pathological damage was severer in groups I/R and Spo than in group S.Compared with group I/R,the levels of BUN,Cr,MDA,TNF-α and IL-6 were significantly decreased,the SOD content was significantly increased (P ＜ 0.05),and the pathological damage was attenuated in group S.Conclusion Sevoflurane postconditioning can reduce the renal injury induced by hind limb I/R in rats,and the reduction of oxygen radical release and inhibition of inflammatory response may be involved in the mechanism.