CAJ | 학술논문

利用ISSR分子标记方法对不同地区的13份水藓科植物进行遗传多样性分析，结果表明，稳定且重复性高的7条引物共扩增出526条清晰的条带，其中518条具有多态性，平均多态性位点比率为98.47％．运用PopGen-32软件分析发现，等位基因数Na=1.7363，有效等位基因数Ne=1.3062，Nei基因的多样性与Shannon信息的指数分别为0.2015和0.3211，说明水藓科植物的遗传多样性比较丰富．13份水藓科植物的遗传分化系数Gst、遗传距离D与遗传一致度I分别为0.0253、0.0071和0.0547，对其进行计算得知，不同属种间存有基因交流，但与种间变异相比，种群内部的遗传变异较大．通过NTSYS2.1软件对13份样本的亲缘关系进行聚类，可将其分为3类，且样本的遗传距离与地理位置有一定的相关性．
이용ISSR분자표기방법대불동지구적13빈수선과식물진행유전다양성분석，결과표명，은정차중복성고적7조인물공확증출526조청석적조대，기중518조구유다태성，평균다태성위점비솔위98.47％．운용PopGen-32연건분석발현，등위기인수Na=1.7363，유효등위기인수Ne=1.3062，Nei기인적다양성여Shannon신식적지수분별위0.2015화0.3211，설명수선과식물적유전다양성비교봉부．13빈수선과식물적유전분화계수Gst、유전거리D여유전일치도I분별위0.0253、0.0071화0.0547，대기진행계산득지，불동속충간존유기인교류，단여충간변이상비，충군내부적유전변이교대．통과NTSYS2.1연건대13빈양본적친연관계진행취류，가장기분위3류，차양본적유전거리여지리위치유일정적상관성．
The genetic diversity of 13 species Fontinalaceae collected in different regions was studied using ISSR marker technique method. The results showed that 526 clear bands were amplified by 7 stable and highly reproducible primers of which 518 were polymorphic. the percentage of polymorphic bands reached 98.47%. The result of analyzed by the software POPGENE-32 showed that number of alleles Na=1.7363, effective number of alleles Ne=1.3062. Nei gene diversity and Shannon information index were 0.201 5 and 0.321 1. It showed that the geographical distribution was very obvious. The coefficient of gene differentiation Gst, genetic distance D and genetic identity I of 13 samples were 0.025 3,0.007 1 and 0.054 7, indicating there was a certain gene flow among different species of Fontinalaceae and the genetic variation was mainly within species compared with interspecies. Cluster analysis among 13 samples genetic relationship using software NTSYS2.1 showed that these samples were divided into there groups. In addition ,there were some correlation between genetic distance and geographic distance among the samples.