CAJ | 학술논문

简述了核酸杂交技术、聚合酶链式反应（PCR）扩增技术、分子指纹技术和克隆基因文库技术4种分子生物学技术，综述了4种技术在海洋、湖泊、河流和其它水生态系统中微生物多样性的研究进展，研究内容大致可分为三个方向：一是人类活动对水环境微生物多样性的影响；二是种群结构和污染之间的规律；三是菌株的筛选和利用，使人们可以在水环境保护、治理过程中变得更加高效、有的放矢。
간술료핵산잡교기술、취합매련식반응（PCR）확증기술、분자지문기술화극륭기인문고기술4충분자생물학기술，종술료4충기술재해양、호박、하류화기타수생태계통중미생물다양성적연구진전，연구내용대치가분위삼개방향：일시인류활동대수배경미생물다양성적영향；이시충군결구화오염지간적규률；삼시균주적사선화이용，사인문가이재수배경보호、치리과정중변득경가고효、유적방시。
This article introduces four kinds of molecular biology technique including nucleic acid hybridization , polymerase chain reaction (PCR ) amplification , molecular fingerprint and clone gene library technology , and summarizes the four techniques'research progress of microbial diversity in different water environment such as the oceans ,lakes ,rivers and other water ecological systems .And then it discusses the research content which can be divided into three aspects :the influence of human activities on water environmental microbial diversity ;the relationship between community structure and pollution ;the utilization and screening of bacterial strain .Based on the research ,it can make the environmental protection and water treatment more efficient and targeted .