CAJ | 학술논문

采用昆仑雪菊水提液灌胃大鼠,以探讨其对大鼠血脂水平的影响.将60只健康成年 SPF 级 SD 大鼠随机分为6组,每组10只.试验前及实验期第28天眼眶采血检测大鼠血清中血脂4项指标：血清胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)与低密度脂蛋白胆固醇(LDL-C).试验结束时处死大鼠并采集大鼠脏器,制作肝组织切片观察雪菊水提液对各组大鼠的肝组织形态变化的影响.试验前各组大鼠的血脂4项、体重指标均无显著差异,使用昆仑雪菊水提液灌胃高脂造模大鼠后,高、中、低3个剂量组与高脂模型组比,TC 含量分别降低了39．0％(P ＜0．01),30．5％(P ＜0．01),32．6％(P ＜0．01)；LDL-C 指标分别降低了40．3％(P ＜0．01),31．0％(P ＜0．05),30．8％(P ＜0．05)；经过统计学分析与正常对照组相比：高脂模型组肝组织切片脂肪空泡明显增多(P ＜0．01),昆仑雪菊干预三个剂量组与高脂模型组肝组织切片比较,脂肪空泡显著减少(P ＜0．05).分析表明,昆仑雪菊水提液可以降低 SD 高脂大鼠模型血脂4项指标中的 TC、LDC-L 的值,且能显著减少 SD 大鼠肝脏的脂肪细胞的数量,说明其具有降血脂的功效.
채용곤륜설국수제액관위대서,이탐토기대대서혈지수평적영향.장60지건강성년 SPF 급 SD 대서수궤분위6조,매조10지.시험전급실험기제28천안광채혈검측대서혈청중혈지4항지표：혈청담고순(TC)、감유삼지(TG)、고밀도지단백담고순(HDL-C)여저밀도지단백담고순(LDL-C).시험결속시처사대서병채집대서장기,제작간조직절편관찰설국수제액대각조대서적간조직형태변화적영향.시험전각조대서적혈지4항、체중지표균무현저차이,사용곤륜설국수제액관위고지조모대서후,고、중、저3개제량조여고지모형조비,TC 함량분별강저료39．0％(P ＜0．01),30．5％(P ＜0．01),32．6％(P ＜0．01)；LDL-C 지표분별강저료40．3％(P ＜0．01),31．0％(P ＜0．05),30．8％(P ＜0．05)；경과통계학분석여정상대조조상비：고지모형조간조직절편지방공포명현증다(P ＜0．01),곤륜설국간예삼개제량조여고지모형조간조직절편비교,지방공포현저감소(P ＜0．05).분석표명,곤륜설국수제액가이강저 SD 고지대서모형혈지4항지표중적 TC、LDC-L 적치,차능현저감소 SD 대서간장적지방세포적수량,설명기구유강혈지적공효.
To investigate the effect of aqueous extract of Coreopsis tinctoria flowers (AECTF)from the Kunlun Mountain on blood fat level in rats,AECTF was administered orally via gavage.Sixty healthy adult SD rats were randomly divided into 6 groups (ten in each group).The four indicators of blood lipids(the to-tal cholesterol (TC),triglycerides (TG),high-density lipoprotein cholesterol (HDL-C)and low density lipoprotein cholesterol (LDL-C)were measured after 28 days of treatment,and liver tissue was collected to observe the effect of AECTF on liver morphology of rats.Result showed that blood fat and bodyweight of rats were similar to each other among the six groups before the treatment.Compared with hyperlipidemia model group,TC content in three AECTF groups (high,medium,low)was decreased by 39.0%,30.5%, 32.6%,respectively,and LDL-C content in three AECTF groups was decreased by 40.3%,31.0%,30.8%, respectively,the differences were significant (P < 0.05).The fat vacuoles of liver tissue were much more in hyperlipidemia model group than that in normal control group (P < 0.01),indicating that hyperlipidemia model was established successfully.The fat vacuoles of liver tissue in three AECTF groups (high,medium, low)were significantly fewer than that in hyperlipidemia model group (P < 0.05).Results suggested that AECTF has a certain effect on decreasing blood fat (TC and LDC-L).Also,AECTF could decrease fat vac-uoles of liver tissue of SD rats.