国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2014年
6期
743-746
,共4页
吴江平%张展东%江沂%苏宁%杨振淮
吳江平%張展東%江沂%囌寧%楊振淮
오강평%장전동%강기%소저%양진회
AO/EB双荧光染色法%蟾酥注射液%细胞凋亡
AO/EB雙熒光染色法%蟾酥註射液%細胞凋亡
AO/EB쌍형광염색법%섬소주사액%세포조망
AO/EB double fluorescent dye staining%Venenum bufonis injection%Apoptosis
目的 验证蟾酥注射液的抗癌活性.方法 将T24细胞、BIU-87细胞分别接种于RPMI1640培养液中37℃孵育,并在多个时间点取细胞悬液加入AO/EB染料染色,在荧光显微镜下观察.结果 在0、3、6、12、24和48 h时,T24细胞的凋亡率分别为3.0％、12.5％、59.5％、66.5％、70.0％和63.5％,BIU-87细胞的凋亡率分别为2.0％、13.5％、55.5％、62.5％、68.5％和64.0％.结论 蟾酥注射液可显著诱导T24细胞、BIU-87细胞凋亡,在体外具有抗癌活性.
目的 驗證蟾酥註射液的抗癌活性.方法 將T24細胞、BIU-87細胞分彆接種于RPMI1640培養液中37℃孵育,併在多箇時間點取細胞懸液加入AO/EB染料染色,在熒光顯微鏡下觀察.結果 在0、3、6、12、24和48 h時,T24細胞的凋亡率分彆為3.0％、12.5％、59.5％、66.5％、70.0％和63.5％,BIU-87細胞的凋亡率分彆為2.0％、13.5％、55.5％、62.5％、68.5％和64.0％.結論 蟾酥註射液可顯著誘導T24細胞、BIU-87細胞凋亡,在體外具有抗癌活性.
목적 험증섬소주사액적항암활성.방법 장T24세포、BIU-87세포분별접충우RPMI1640배양액중37℃부육,병재다개시간점취세포현액가입AO/EB염료염색,재형광현미경하관찰.결과 재0、3、6、12、24화48 h시,T24세포적조망솔분별위3.0％、12.5％、59.5％、66.5％、70.0％화63.5％,BIU-87세포적조망솔분별위2.0％、13.5％、55.5％、62.5％、68.5％화64.0％.결론 섬소주사액가현저유도T24세포、BIU-87세포조망,재체외구유항암활성.
Objective To determine the anticancer activity of venenum bufonis injection.Methods T24 and BIU-87 cells were seeded in RPMI 1640 culture medium and incubated at 37 ℃.Cell suspension was taken at multiple time points and stained by AO/EB dye and then was observed under fluorescence microscope.Results At hour 0,3,6,12,24,and 48,the apoptosis rates of T24 cells were 3.0％,12.5％,59.5％,66.5％,70.0％,and 63.5％ respectively; and the apoptosis rates of BIU-87 cells were 2.0％,13.5％,55.5％,62.5％,68.5％,and 64.0％,respectively.Conclusions The apoptosis of T24 and BIU-87 cells can be obviously induced by venenum bufonis injection.Venenum bufonis injection has anticancer activity in vitro.
