CAJ | 학술논문

目的：探讨红景天苷对人成骨肉瘤细胞株 MG63诱导成骨分化作用及相关分子机制。方法 MTT 法测定不同浓度红景天苷对 MG63细胞增殖活力的影响；PNPP 法检测经红景天苷处理后成骨性标志物--碱性磷酸酶活性的变化；RT-PCR 检测细胞内 bmp2 mRNA 水平的变化；Western blot 及流式细胞术检测给药前后细胞内 BMP2、IκBα蛋白表达水平的变化。结果红景天苷能显著促进 MG63细胞的增殖并上调其ALP 活性，其对ALP 活性的上调呈现一定的时间和剂量依赖性，其中在5μmol/L、48 h 条件下表现出最佳效果，证明红景天苷具有促成骨分化作用。此外，红景天苷能显著上调细胞内 bmp2 mRNA 及蛋白表达水平，并能时间依赖性激活 IκBα，抑制 NF-κB 活性。结论红景天苷能促进人成骨肉瘤细胞成骨分化，具有潜在的骨代谢调节作用，其作用机制可能与抑制 NF-κB 活性并激活 BMP2信号通路有关。
목적：탐토홍경천감대인성골육류세포주 MG63유도성골분화작용급상관분자궤제。방법 MTT 법측정불동농도홍경천감대 MG63세포증식활력적영향；PNPP 법검측경홍경천감처리후성골성표지물--감성린산매활성적변화；RT-PCR 검측세포내 bmp2 mRNA 수평적변화；Western blot 급류식세포술검측급약전후세포내 BMP2、IκBα단백표체수평적변화。결과홍경천감능현저촉진 MG63세포적증식병상조기ALP 활성，기대ALP 활성적상조정현일정적시간화제량의뢰성，기중재5μmol/L、48 h 조건하표현출최가효과，증명홍경천감구유촉성골분화작용。차외，홍경천감능현저상조세포내 bmp2 mRNA 급단백표체수평，병능시간의뢰성격활 IκBα，억제 NF-κB 활성。결론홍경천감능촉진인성골육류세포성골분화，구유잠재적골대사조절작용，기작용궤제가능여억제 NF-κB 활성병격활 BMP2신호통로유관。
Objective To investigate the effect of salidroside (SAL) on osteoblast differentiation and related signaling factors in human osteosarcoma cells MG63. Methods Cell viability was assessed by MTT method, and the ALP activity was measured by PNPP method. Gene expression of bmp2 was detected by RT-PCR analysis, and the protein levels of BMP2 and IκBα were determined by Western blot and flow cytometry. Results SAL promoted the proliferation of MG63 cells. In addition, the ALP activity was significantly increased in a time- and dose-dependent manner after SAL treatment, suggesting that SAL could stimulate osteoblast differentiation. Both the bmp2 mRNA and protein expressions were increased after SAL treatment. Meanwhile, SAL could time-dependently increase IκBα expression, which may affect NF-κB activity. Conclusion SAL can stimulate osteoblast differentiation and regulate both NF-κB and BMP2 signaling pathway in MG63 cells, suggesting that the natural product may be developed as a promising drug with little toxicity to regulate bone metabolism.