CAJ | 학술논문

目的研究舒张性心力衰竭大鼠左心室基质金属蛋白酶-2、9的变化。方法34只雄性SD大鼠随机分为实验组和对照组（n=17），实验组建立两肾一夹模型，对照组行假手术。术后12周时左心导管插管法测定血流动力学指标、左心室质量指数；采用ELISA及免疫组化法测定左心室组织MMP-2、9的蛋白表达。结果与对照组比较，实验组收缩压，平均颈动脉压分别升高24.69%、32.68%（均P＜0.01），而左心室质量指数和LVEDP升高38.38%和418.56%（P＜0.05或0.01）；+dp/dtmax及- dp/dtmax升高8.56%（P＜0.05）和11.34%（P＜0.01）。ELISA法测定左心室组织MMP-2、9分别较对照组增高29.8%、7.95%（均P＜0.01）；免疫组化累积光密度显示实验组左心室组织MMP-2、9分别较对照组增高132.61%、29.71%（均P＜0.01）。结论舒张性心力衰竭大鼠左心室MMP-2、9合成增加。
목적연구서장성심력쇠갈대서좌심실기질금속단백매-2、9적변화。방법34지웅성SD대서수궤분위실험조화대조조（n=17），실험조건립량신일협모형，대조조행가수술。술후12주시좌심도관삽관법측정혈류동역학지표、좌심실질량지수；채용ELISA급면역조화법측정좌심실조직MMP-2、9적단백표체。결과여대조조비교，실험조수축압，평균경동맥압분별승고24.69%、32.68%（균P＜0.01），이좌심실질량지수화LVEDP승고38.38%화418.56%（P＜0.05혹0.01）；+dp/dtmax급- dp/dtmax승고8.56%（P＜0.05）화11.34%（P＜0.01）。ELISA법측정좌심실조직MMP-2、9분별교대조조증고29.8%、7.95%（균P＜0.01）；면역조화루적광밀도현시실험조좌심실조직MMP-2、9분별교대조조증고132.61%、29.71%（균P＜0.01）。결론서장성심력쇠갈대서좌심실MMP-2、9합성증가。
Objective To explore the changes of matrix metalloproteinase(MMP)- 2, 9 in left ventricle (LV)of rats with diastolic heart failure. Methods Thirty- four male Sprague- Dawley rats were randomly divided into normal control group (n=17)and model group (n=17). One renal artery was ligated in model group and sham operation was done in control group. 12 weeks later,LV hemodynamics was determined by a cardiac catheter and the ratio of LV mass (LVM) to body weight(BW) was measured. MMP- 2, 9 of LV myocardium were measured by enzyme linked immunosorbent assay(ELISA) and immunohistochemistry(IHC). Results The systolic blood pressure and carotid artery pressure increased by 24.69%and 32.68%(al P<0.01), LVM/BW and LV end diastolic pressure increased by 38.38% and 418.56%(P<0.05 or 0.01),+dp/dt max increased by 8.56%(P<0.05), and- dp/dt max decreased by 11.34%(P<0.01) in model group than in control group. The expression of MMP- 2、9 of LV myocardium increased by 29.8% and 7.95% on ELISA or by 132.61%and 29.71%on IHC, respectively, in model group than in control group (al P<0.01). Conclusion MMP- 2、9 synthesis increases in LV myocardium of rats with diastolic heart failure.