CAJ | 학술논문

目的：观察氟比洛芬酯注射液（flurbiprofen axetil injection ，FAI）对腰椎间盘突出症兔椎间盘髓核组织中磷脂酶 A2（PLA2）、前列腺素 E2（PGE2）水平的影响，探讨 FAI对腰椎间盘突出症的治疗作用及其机制。方法：雄性6月龄新西兰大白兔，于L3-4和L4-5节段椎间盘用18 G 针头行纤维环穿刺，制作兔腰椎间盘突出症动物模型。术后3周行计算机断层扫描（CT ）检查，确认造模成功32只，随机分为治疗组和对照组，每组16只。采用硬膜外注射给药，治疗组注射 FAI ，对照组注射等量生理盐水。比较治疗前后的自由行走痛行为学评分；治疗结束后第7天处死动物，获取椎间盘组织，ELISA 法测定其中的 PLA2、PGE2水平，HE 染色行形态学学观察。结果：与对照组比较，治疗后痛行为学评分治疗组显著下降（P＜0．05），治疗组椎间盘髓核组织中 PLA2和 PGE2水平显著降低（P＜0．05）；HE染色显示：对照组椎间盘髓核及纤维环组织内可见肉芽组织生长，并有大量炎性细胞浸润；治疗组椎间盘髓核及纤维环组织内无肉芽组织生长及炎性细胞浸润。结论：FAI能显著降低腰椎间盘突出症兔椎间盘髓核组织中 PLA2和 PGE2水平，降低腰椎间盘组织病理损伤，在椎间盘突出症的治疗中发挥抗炎镇痛作用。
목적：관찰불비락분지주사액（flurbiprofen axetil injection ，FAI）대요추간반돌출증토추간반수핵조직중린지매 A2（PLA2）、전렬선소 E2（PGE2）수평적영향，탐토 FAI대요추간반돌출증적치료작용급기궤제。방법：웅성6월령신서란대백토，우L3-4화L4-5절단추간반용18 G 침두행섬유배천자，제작토요추간반돌출증동물모형。술후3주행계산궤단층소묘（CT ）검사，학인조모성공32지，수궤분위치료조화대조조，매조16지。채용경막외주사급약，치료조주사 FAI ，대조조주사등량생리염수。비교치료전후적자유행주통행위학평분；치료결속후제7천처사동물，획취추간반조직，ELISA 법측정기중적 PLA2、PGE2수평，HE 염색행형태학학관찰。결과：여대조조비교，치료후통행위학평분치료조현저하강（P＜0．05），치료조추간반수핵조직중 PLA2화 PGE2수평현저강저（P＜0．05）；HE염색현시：대조조추간반수핵급섬유배조직내가견육아조직생장，병유대량염성세포침윤；치료조추간반수핵급섬유배조직내무육아조직생장급염성세포침윤。결론：FAI능현저강저요추간반돌출증토추간반수핵조직중 PLA2화 PGE2수평，강저요추간반조직병리손상，재추간반돌출증적치료중발휘항염진통작용。
Objective:To study the effects of flurbiprofen axetil injection (FAI) on phospholipase A2 (PLA2) and prostaglandin E2(PGE2) level of rabbit's lubar intervertebral disc ,and to explore the mechanism of FAI in treating lubar intervertebral disc .Methods :6 month-old male New Zealand w hite rabbits ,to be made for the animal model of rabbit's lumbar intervertebral disc protrusion (LIDP) by fiber ring centesis in L3-4 and L4-5 ;and all rabbits were studied with computed tomography (CT ) 3 weeks postoperatively .After animal models were made successful ,32 rabbits were divided into 2 groups randomly ,with 16 rabbits in each group .The observation group was injected outside putamen with FAI ,the control group was injected with physiological saline equally .Then we observed the changes of activities and pain behaviors of rabbits through pain scores before and after treatment .All the rabbit-models were sacrificed 7days after treatment ,the disc tissues were examined pathologically by HE dying and ELISA (PLA2、PGE2) .Results :The pain scores and PLA2 ,PGE2 of obsevation group fell obviously compared with control group (P<0 .05) .But the grow th of granulation tissue in nucleus pulposus and fibrous ring were observed by HE dying in control group .Conclu-sion:FAI could lower the expression of PLA2 and PGE2 significantly ,reduces the injuring of lumbar inter-vertebral disc .So it has effects of anti-inflammation and analgesia .